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Similar Differentiation Patterns Between PBP Expression Levels and Pheromone Component Ratios in Two Populations of Sesamia nonagrioides

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Abstract

Pheromone-binding proteins (PBPs) are thought to contribute to the specificity of the pheromone detection system through an initial selective binding with pheromone molecules. Here, we report different expression levels of PBP transcripts in the antennae of two populations of the stemborer Sesamia nonagrioides (Lepidoptera: Noctuidae), one collected in Europe and one in sub-Saharan Africa. The three PBP transcripts previously identified in this species were found to be expressed in both male and female antennae. Whereas PBP3 did not show any differential expression, PBP1 and PBP2 appeared to be expressed differently according to the population origin and sex. Simultaneously, we measured and compared the ratio of the three components of the S. nonagrioides pheromone blend (Z11-16:Ac; Z11-16:OH; Z11–16:Ald) in females of the two populations. The ratio of Z11-16:OH and Z11–16:Ald varied significantly according to the population origin of this species. Cluster analyses revealed similar differentiation patterns between PBP1 and PBP2 expression levels and the ratios of Z11-16:OH and Z11–16:Ald. Different female sexual signals may thus correspond to different male reception systems, which are adjusted by the PBP expression levels, thereby ensuring optimal communication within populations.

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Acknowledgments

Thanks are given to Erick Campan and Alban Sagouis from the Paul Sabatier University (Toulouse, France) and to Floriane Chardonnet and Nicolas Joly (LEGS c/o CNRS, Gif-sur-Yvette, France) for help in collecting the French population of S. nonagrioides and the IRD—NSBB (Noctuid Stem Borer Biodiversity) team, particularly Gerphas Okuku, at ICIPE (Nairobi, Kenya) for collection of the African population of S. nonagrioides. We also thank Ferial Kaoula (LEGS c/o CNRS, Gif-sur-Yvette, France) for insect rearing and Fritz Schulthess for critical reading of the manuscript and his corrections to the English. This work was supported by funding from the French ANR (Agence Nationale de la Recherche) (Adaptanthrop project ANR-09-PEXT-009).

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Authors and Affiliations

  1. IRD, UR 072, c/o CNRS, Laboratoire Evolution, Génomes et Spéciation, 91198, Gif-sur-Yvette Cedex, France

    Nicolas Glaser, Claire Capdevielle-Dulac, Bruno Le Ru, Myriam Harry & Paul-Andre Calatayud

  2. INRA, Institute of Ecology and Environmental Sciences iEES-Paris, Route de Saint-Cyr, 78026, Versailles Cedex, France

    Nicolas Glaser, Brigitte Frérot, Ene Leppik, Christelle Monsempes & Emmanuelle Jacquin-Joly

  3. Université Paris-Sud, 91405, Orsay Cedex, France

    Claire Capdevielle-Dulac, Bruno Le Ru, Myriam Harry & Paul-Andre Calatayud

  4. IRD, UR 072, c/o icipe, NSBB Project, PO Box 30772-00100, Nairobi, Kenya

    Bruno Le Ru & Paul-Andre Calatayud

  5. Laboratoire de Zoologie, Research institute of Biosciences, University of Mons, Place du Parc 20, 7000, Mons, Belgium

    Thomas Lecocq

Authors
  1. Nicolas Glaser
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  2. Brigitte Frérot
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  3. Ene Leppik
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  4. Christelle Monsempes
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  5. Claire Capdevielle-Dulac
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  6. Bruno Le Ru
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  7. Thomas Lecocq
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  8. Myriam Harry
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  9. Emmanuelle Jacquin-Joly
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  10. Paul-Andre Calatayud
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Correspondence to Paul-Andre Calatayud.

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Glaser, N., Frérot, B., Leppik, E. et al. Similar Differentiation Patterns Between PBP Expression Levels and Pheromone Component Ratios in Two Populations of Sesamia nonagrioides . J Chem Ecol 40, 923–927 (2014). https://doi.org/10.1007/s10886-014-0485-2

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  • DOI: https://doi.org/10.1007/s10886-014-0485-2

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