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Lysophospholipids in the Mediterranean Sponge Oscarella tuberculata: Seasonal Variability and Putative Biological Role

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Abstract

Lysophospholipids (LPLs) are recognized as important signaling molecules in metazoan cells. LPLs seem to be widely distributed among marine invertebrates, but their physiological role remains poorly known. Marine sponges produce original phospholipids and LPLs whose isolation and structural elucidation rarely have been reported. Two LPLs were isolated for the first time from the Mediterranean Homoscleromorph sponge Oscarella tuberculata: a bioactive lyso-PAF already identified in some other sponge species; and the new lysophosphatidylethanolamine C20:2 (LPE 1). The expression of LPL metabolites was investigated over time to determine their baseline variations and to relate them to the sponge reproduction pattern in order to better understand their putative role in the sponge life cycle. Expression levels of both compounds appeared to be highly correlated displaying significant seasonal fluctuations with maximal values in summer and minimal in winter. A significant higher LPL content was detected in reproductive sponges and especially in females, with a peak occurring during embryogenesis and larval development. The results suggest that LPLs could play a role of mediators in sponge embryogenesis and morphogenesis.

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Acknowledgments

This study was funded by ECIMAR project (ANR-06-BDIV-001), and partly by the “Institut de Chimie des Substances Naturelles (ICSN)” and the Pôle Mer PACA. We thank M. Gaysinski and the PFTC of Nice for recording the NMR spectra and J.-M. Guigonis for recording the HRMS spectra. We acknowledge the assistance of Ainara Gonzalez for the study of the sponge life cycle and I. Florent (Muséum National d’Histoire Naturelle de Paris) for the antimalarial bioassay, and the ICSN for the antitumoral assay.

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Authors and Affiliations

  1. Centre d’Océanologie de Marseille, Diversité, Evolution et Ecologie Fonctionnelle Marine, Université de la Méditérranée, UMR 6540 CNRS, Rue de la Batterie des Lions, 13007, Marseille, France

    Julijana Ivanisevic, Thierry Pérez & Alexander V. Ereskovsky

  2. Laboratoire de Chimie des Molécules Bioactives et des Arômes, Université de Nice-Sophia Antipolis, UMR 6001 CNRS, Parc Valrose, 06108, Nice, France

    Julijana Ivanisevic & Olivier P. Thomas

  3. Pôle Mer et Littoral—UPRES-EA 2160, Groupe Substances marines à activité biologique, Laboratoire de Chimie marine—ISOMer, 2 rue La Houssinière, 44322, Nantes, France

    Gilles Barnathan

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  1. Julijana Ivanisevic
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  2. Thierry Pérez
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Correspondence to Thierry Pérez.

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Ivanisevic, J., Pérez, T., Ereskovsky, A.V. et al. Lysophospholipids in the Mediterranean Sponge Oscarella tuberculata: Seasonal Variability and Putative Biological Role. J Chem Ecol 37, 537–545 (2011). https://doi.org/10.1007/s10886-011-9943-2

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  • DOI: https://doi.org/10.1007/s10886-011-9943-2

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