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Volatiles Mediating a Plant-Herbivore-Natural Enemy Interaction in Resistant and Susceptible Soybean Cultivars

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Abstract

Several studies have shown that herbivore-induced plant volatiles act directly on herbivores and indirectly on their natural enemies. However, little is known about the effect of herbivore damage on resistant and susceptible plant cultivars and its effect on their natural enemies. Thus, the aim of this study was to evaluate the attraction of the herbivorous pentatomid bug Euschistus heros and its egg parasitoid Telenomus podisi to two resistant and one susceptible soybean cultivars with different types of damage (herbivory, herbivory+oviposition, and oviposition). In a Y-tube olfactometer, the parasitoids were attracted to herbivory and herbivory+oviposition damaged soybean plants when compared to undamaged soybean plants for the resistant cultivars, but did not show preference for the susceptible cultivar Silvânia in any of the damage treatments. The plant volatiles emitted by oviposition-damaged plants in the three cultivars did not attract the egg parasitoid. In four-arm-olfactometer bioassays, E. heros females did not show preference for odors of damaged or undamaged soybean plants of the three cultivars studied. The Principal Response Curves (PRC) analysis showed consistent variability over time in the chemical profile of volatiles between treatments for the resistant cultivar Dowling. The compounds that most contributed to the divergence between damaged soybean plants compared to undamaged plants were (E,E)-α-farnesene, methyl salicylate, (Z)-3-hexenyl acetate, and (E)-2-octen-1-ol.

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Acknowledgements

We thank H. Santos and D. Tiburcio for helping with laboratory rearing of the insects, and Jeffrey Aldrich and Michael Birkett for providing synthetic standards. We are grateful to Dr. Mark Horn International Consultant at Embrapa Genetic Resources and Biotechnology, to Monika Hilker and Martin Pareja for comments that improved the manuscript and for revising the English. We also thank the Post-Graduate Animal Biology program of University of Brasília (UnB) for use of their facility. This work received financial support from the Brazilian Council for Scientific and Technological Development (CNPq) through a grant to Mirian F.F. Michereff, International Foundation for Science IFS, Distrito Federal Research Foundation (FAP-DF) and Brazilian Corporation of Agricultural Research (EMBRAPA).

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Authors and Affiliations

  1. Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil

    Mirian Fernandes Furtado Michereff, Raúl Alberto Laumann, Miguel Borges & Maria Carolina Blassioli Moraes

  2. Departamento de Zoologia, Universidade de Brasília, Brasília, Brazil

    Mirian Fernandes Furtado Michereff & Ivone Rezende Diniz

  3. Embrapa Hortaliças, Brasília, Brazil

    Miguel Michereff-Filho

  4. Embrapa Cerrados, Brasília, Brazil

    Austeclínio Lopes Farias Neto

  5. Embrapa Recursos Genéticos e Biotecnologia-Parque Estação Biológica-W5 Norte, CEP 70770-900, Brasília, DF, Brazil

    Maria Carolina Blassioli Moraes

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  1. Mirian Fernandes Furtado Michereff
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Correspondence to Maria Carolina Blassioli Moraes.

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Michereff, M.F.F., Laumann, R.A., Borges, M. et al. Volatiles Mediating a Plant-Herbivore-Natural Enemy Interaction in Resistant and Susceptible Soybean Cultivars. J Chem Ecol 37, 273–285 (2011). https://doi.org/10.1007/s10886-011-9917-4

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