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Natural Ligands of Porcine Olfactory Binding Proteins

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Abstract

Knowledge of endogenous ligands of olfactory binding proteins is a prerequisite for studying their role in odor and pheromone transduction. Here, we report the extraction, derivatization, and characterization by gas chromatography-mass spectrometry of the natural ligands of pig, Sus scrofa (L.), Von Ebner’s Gland protein (VEG) and odorant binding protein (OBP). We identified two isoforms (VEG1 and VEG2), which differed only by the linkage of an O-N-acetylglucosamine (O-GlcNac) group on VEG1. The natural ligands of VEG1 were characterized as two isomers of testosterone, whereas ligands of VEG2 and OBP were fatty acids or their derivatives. Our findings suggest that the binding specificity of VEG1 for steroids is governed by the presence of an O-GlcNac moiety on the protein. This specificity was confirmed by the binding of radiolabeled testosterone only by VEG1 in an in-gel binding assay. This is the first evidence for a post-translational modification in the process of odorant discrimination by olfactory binding proteins.

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Authors and Affiliations

  1. INRA, UMR 8576 CNRS/Université Lille1, Unité de Glycobiologie Structurale et Fonctionnelle, 59655, Villeneuve d’Ascq CEDEX, France

    Chrystelle Le Danvic, Djamel Abderrahmani & Patricia Nagnan-Le Meillour

  2. Université de Provence, UMR 6149 CNRS/Université de Provence, Laboratoire de Neurobiologie Intégrative et Adaptative, 3 place Victor Hugo, 13331, Marseille CEDEX 03, France

    Gaelle Guiraudie-Capraz

  3. CNRS, UMR 8576 CNRS/Université Lille1, Unité de Glycobiologie Structurale et Fonctionnelle, 59655, Villeneuve d’Ascq CEDEX, France

    Jean-Pierre Zanetta

Authors
  1. Chrystelle Le Danvic
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  2. Gaelle Guiraudie-Capraz
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  3. Djamel Abderrahmani
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  4. Jean-Pierre Zanetta
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  5. Patricia Nagnan-Le Meillour
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Correspondence to Patricia Nagnan-Le Meillour.

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Le Danvic, C., Guiraudie-Capraz, G., Abderrahmani, D. et al. Natural Ligands of Porcine Olfactory Binding Proteins. J Chem Ecol 35, 741–751 (2009). https://doi.org/10.1007/s10886-009-9645-1

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  • DOI: https://doi.org/10.1007/s10886-009-9645-1

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