Abstract
A previous flight tunnel study showed that 3–5% of European corn borer (ECB) moths, Ostrinia nubilalis (Z/E11-14:OAc), could fly upwind and make contact with sources releasing the sex pheromone of the related Asian corn borer (ACB), Ostrinia furnacalis (2:1 Z/E12-14:OAc). In this study, we show that rare males (3–4%) are also present in South Korean ACB that respond to the sex pheromone blends of the ECB UZ (97:3 Z/E11-14:OAc) and BE (1:99 Z/E11-14:OAc) pheromone races. We also show that the upwind flight response of a significant proportion of male ACB was antagonized by the addition of 1% Z9-14:OAc to the ACB blend, a compound that also antagonizes the upwind flight of ECB males. Male ACB flight behavior was not, however, affected by adding either of the ECB blends to the ACB blend, or by the addition of 50% 14:OAc, a compound identified from female pheromone glands of ACB and a number of other Ostrinia species. Additional flight tunnel tests with ACB to study the comparative aspects of ECB and ACB pheromone response specificity showed that male ACB exhibited maximal levels of upwind flight and source contact with doses of pheromone (30 and 100 μg on rubber septum sources) that also elicited maximal levels in the two ECB pheromone races. The maximal level of source contact for ACB (66%) was lower than observed with the UZ race of ECB to its pheromone blend (>95%), but comparable to those for the BE race of ECB (65–70%). Male ACB also flew upwind in high proportions to a broader range of ratios of Z/E12-14:OAc (80:20 to 20:80) than was previously observed for either of the ECB races.
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Ando, T., Saito, O., Arai, K., and Takahashi, N. 1980. (Z)- and (E)-12-Tetradecenyl acetates: sex pheromone components of oriental corn borer (Lepidoptera: Pyralidae). Agric. Biol. Chem. 44:2643–2649.
Baker, T. C. 2002. Mechanism for saltational shifts in pheromone communication systems. Proc. Natl. Acad. Sci. USA 99:13368–13370.
Boo, K. S. and Park, J. W. 1998. Sex pheromone of the Asian corn borer moth, Ostrinia furnacalis (Guenée) (Lepidoptera: Pyralidae) in South Korea. J. Asia Pacific Entomol. 1:77–84.
Cheng, Z. Q., Xiao, J. C., Huang, X. T., Chen, D. L., Li, Q. J., Li, Y. S., Huang, S. R., Luo, Q. C., Yang, C. M., and Yang, T. H. 1981. Sex pheromone components isolated from China corn borer, Ostrinia furnacalis, Guenée (Lepidoptera: Pyralidae), (E)- and (Z)-12-tetradecenyl acetates. J. Chem. Ecol. 7:841–851.
Cossé, A. A., Campbell, M. G., Glover, T. J., Linn, C. E. Jr., Todd, J. L., Baker, T. C., and Roelofs, W. L. 1995. Pheromone behavioral responses in unusual male European corn borer hybrid progeny not correlated to electrophysiological phenotypes of their pheromone-specific antennal neurons. Experientia 51:809–816.
Domingue, M. J., Roelofs, W. L., Linn, C. E. Jr., and Baker, T. C. 2006. Effects of egg-to-adult development time and age on olfactory neuron response to semiochemicals in European corn borers. J. Insect Physiol. 52:975–983.
Du, J. W., Zhu, T. P., Zhang, T. P., Xu, S. F., and Dai, X. J. 1986. Studies on the precise blending of the pheromone components of the Asian corn borer, Ostrinia furnacalis Guenée (Lep. Pyralidae). Contrib. Shanghai Inst. Entomol. 6:17–22.
Glover, T. J., Perez, N., and Roelofs, W. L. 1989. Comparative analysis of sex-pheromone-response antagonists in three races of European corn borer. J. Chem. Ecol. 15:863–873.
Huang, Y., Takanashi, T., Hoshizaki, S., Tatsuki, S., Honda, H., Yoshiyasu, Y., and Ishikawa, Y. 1998. Geographic variation in the sex pheromone of Asian corn borer, Ostrinia furnacalis, in Japan. J. Chem. Ecol. 24:2079–2088.
Huang, Y., Takanashi, T., Hoshizaki, S., Tatsuki, S., and Ishikawa, Y. 2002. Female sex pheromone polymorphism in adzuki bean borer, Ostrinia scapulalis, is similar to that in European corn borer, O. nubilalis. J. Chem. Ecol. 28:533–539.
Ishikawa, Y., Takanashi, T., Kim, C.-G., Hoshizaki, S., Tatsuki, S., and Huang, Y. 1999a. Ostrinia spp. in Japan: their host plants and sex pheromones. Entomol. Exp. Appl. 91:237–244.
Ishikawa, Y., Takanashi, T., and Huang, Y. 1999b. Comparative studies on the sex pheromones of Ostrinia spp. in Japan: the burdock borer, Ostrinia zealis. Chemoecology 9:25–32.
Kim, C., Hoshizaki, S., Huang, Y., Tatsuki, S., and Ishikawa, Y. 1999. Usefulness of mitochondrial COII gene sequences in examining phylogenetic relationships in the Asian corn borer, Ostrinia furnacalis, and allied species (Lepidoptera: Pyralidae). Appl. Entomol. Zool. 34:405–412.
Klun, J. A., Bierl-Leonhardt, B. A., Schwarz, M., Litsinger, J. A. Barrion, A. T., Chiang, H. C., and Jiang, Z. 1980. Sex pheromone of the Asian corn borer moth. Life Sci. 27:1603–1606.
Kochansky, J., Cardé R. T., Liebherr, J., and Roelofs, W. L. 1975. Sex pheromones of the European corn borer in New York. J. Chem. Ecol. 1:225–231.
Kou, R., Ho, H. Y., Yang, H. T., Chow, Y. S., and Wu, H. J. 1992. Investigation of sex pheromone components of female Asian corn borer, Ostrinia furnacalis (Hübner) (Lepidoptera: Pyralidae) in Taiwan. J. Chem. Ecol. 18:833–840.
Linn, C. E. Jr. and Roelofs, W. L. 1995. Pheromone communication in the moths and its role in the speciation process, pp. 263–300, in D. Lambert and H. Spencer (eds.). Speciation and the Recognition Concept: Theory and Application. Johns Hopkins University Press, Baltimore, MD.
Linn, C. E. Jr., Young, M. S., Gendle, M., Glover, T. J., and Roelofs W. L. 1997. Sex pheromone blend discrimination in two races and hybrids of the European corn borer moth, Ostrinia nubilalis. Physiol. Entomol. 22:212–223.
Linn, C. E. Jr., O’connor, M., and Roelofs, W. L. 2003. Silent genes and rare males: a fresh look at pheromone blend response specificity in the European corn borer moth, Ostrinia nubilalis. J. Insect Sci. 3:15 (Available online: http://www.insectscience.org/3.15).
Löfstedt, C., Hansson, B. S., Roelofs, W. L., and Bengtsson, B. O. 1989. No linkage between genes controlling female pheromone production and male pheromone response in the European corn borer Ostrinia nubilalis Hübner (Lepidoptera: Pyralidae). Genetics 123:553–556.
Mutuura, A. and Munroe, E. 1970. Taxonomy and distribution of the European corn borer and allied species: genus Ostrinia (Lepidoptera: Pyralidae). Mem. Entomol. Soc. Can. 71:1–112.
Roelofs, W. L., Du, J. W., Tang, X. H., Robbins, P. S., and Eckenrode, C. J. 1985. Three European corn borer populations in New York based on sex pheromones and voltinism. J. Chem. Ecol. 11:829–836.
Roelofs, W. L., Glover, T. J., Tang, X.-H., Sreng, I., Robbins, P., Eckenrode, C. J., Löfstedt, C., Hansson, B. S., and Bengtsson, B. O. 1987. Sex pheromone production and perception in European corn borer moths requires both autosomal and sex-linked genes. Proc. Natl. Acad. Sci. USA 84:7585–7589.
Roelofs, W. L., Liu, W., Hao, G., Jiao, H., Rooney, A. P., and Linn, C. E. Jr. 2002. Evolution of moth sex pheromones via ancestral genes. Proc. Natl. Acad. Sci. USA 99:13621–13626.
Yeh, S., Lee, K., Chang, K.-T., Yen, F.-C., and Hwang, J. S. 1989. Sex pheromone components from Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae) in Taiwan. J. Chem. Ecol. 15:497–505.
Zhao, C.-H., Lu, F., Bengtsson, M., and Löfstedt, C. 1995. Substrate specificity of acetyltransferase and reductase enzyme systems used in pheromone biosynthesis by Asian corn borer, Ostrinia furnacalis. J. Chem. Ecol. 21:1495–1509.
Acknowledgements
We thank Kathy Poole for help in maintaining the ACB and ECB colonies, and Satoshi Nojima and Shannon Olsson for preparing the solutions for flight tunnel tests. The project was funded by NSF IBN #034340.
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Linn, C.E., Musto, C.J. & Roelofs, W.L. More Rare Males in Ostrinia: Response of Asian Corn Borer Moths to the Sex Pheromone of the European Corn Borer. J Chem Ecol 33, 199–212 (2007). https://doi.org/10.1007/s10886-006-9204-y
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DOI: https://doi.org/10.1007/s10886-006-9204-y