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Mannose-Binding Lectin Levels and Variation During Invasive Candidiasis

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Abstract

The high morbi-mortality associated with invasive candidiasis (IC) is a persistent problem in hospitals. Mannose-binding lectin (MBL) plays a role in innate immunity through its interaction with mannosylated molecules of Candida albicans. A correlation between MBL deficiency and vulvovaginal candidiasis or peritonitis has been reported. We investigated circulating MBL levels and their evolution during the course of IC. Sixty-eight patients with proven IC, 82 hospitalized patients (HP) without evidence of infection, and 70 healthy subjects (HS) were studied in order to examine the relationship between serum MBL and IC. Serum MBL levels were measured by enzyme-linked immunosorbent assay (ELISA). MBL levels were significantly higher in IC patients than in HP and HS (p < 0.0001, p < 0.0055, respectively). A change in MBL concentrations was observed during the course of IC, with a dramatic decrease during the 2 days before positive blood culture sampling. This decrease was concomitant with the presence of high levels of circulating mannan (Mn). Like MBL levels, anti-mannan antibodies (AMn) increased after the mannanemia/blood culture period. These findings suggest a possible role of MBL during the early stage of IC. The mechanisms that regulate these observations in terms of effect and consequences on innate and adaptive immunity and the prognosis of IC require further investigation.

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Acknowledgements

This work was supported by a grant from the “Programme Hospitalier de Recherche Clinique du Ministère des Affaires Sociales, de la Santé et de la Ville.″ PHRC 1918, 2011-10-27, by INSERM, and by the European Community’s Seventh Framework Program (FP7-2007-2013) under grant agreement no. HEALTH-F2-2010-260338 ‘ALLFUN’. We thank Dr Val Hopwood for editing the manuscript.

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All authors have no commercial relationship or conflict of interest of any nature related to the present study.

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Authors and Affiliations

  1. Université Lille Nord de France, Lille Cedex, France

    Sébastien Damiens, Julien Poissy, Samir Jawhara, Thierry Jouault, Daniel Poulain & Boualem Sendid

  2. UDSL, Lille Cedex, France

    Sébastien Damiens, Julien Poissy, Samir Jawhara, Thierry Jouault, Daniel Poulain & Boualem Sendid

  3. Unité INSERM 995-2, Université Lille 2, Group “Régulations de l’interface glycanique Candida/hôte”, Pôle Recherche, Faculté de Médecine, Place Verdun, 59045, Lille Cedex, France

    Sébastien Damiens, Julien Poissy, Samir Jawhara, Thierry Jouault, Daniel Poulain & Boualem Sendid

  4. EA2694, Unité de Biostatistiques, Lille Cedex, France

    Julia Salleron

  5. Service de Parasitologie Mycologie, Institut de Microbiologie, CHRU Lille, Lille Cedex, France

    Sébastien Damiens, Nadine François, Daniel Poulain & Boualem Sendid

  6. Pôle de Réanimation, CHRU Lille, Lille Cedex, France

    Julien Poissy

Authors
  1. Sébastien Damiens
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  2. Julien Poissy
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  3. Nadine François
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  4. Julia Salleron
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  5. Samir Jawhara
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  6. Thierry Jouault
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  7. Daniel Poulain
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  8. Boualem Sendid
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Correspondence to Boualem Sendid.

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Damiens, S., Poissy, J., François, N. et al. Mannose-Binding Lectin Levels and Variation During Invasive Candidiasis. J Clin Immunol 32, 1317–1323 (2012). https://doi.org/10.1007/s10875-012-9748-2

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  • DOI: https://doi.org/10.1007/s10875-012-9748-2

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