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Increased B7-H1 Expression on Peripheral Blood T Cells in Oral Lichen Planus Correlated with Disease Severity

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Abstract

Background

Oral lichen planus (OLP) is a chronic and T cell-mediated autoimmune disease whose immunopathogenesis may involve antigen-presentation, T cells activation and migration as well as keratinocytes apoptosis. PD-1/B7-H1 pathway may have a unique function in regulating self-reactive T cells associated with inflammatory response and maintaining tolerance in peripheral tissues. In this study, we aimed to explore the contribution of PD-1/B7-H1 pathway to OLP.

Methods

We determined the expression of PD-1 and B7-H1 on peripheral blood T cells from OLP cases and analyzed their association with disease severity assessed by RAE (reticular, atrophic and erosive lesion) scoring system. In addition, interferon-γ, interleukin (IL)-2, IL-4, IL-10 and soluble PD-1 concentrations in serum were measured using ELISA. Then, we explored the regulation of PD-1/B7-H1 pathway on T cells immune response in OLP by blockade of PD-1 or B7-H1.

Results

We found that PD-1 and B7-H1 were up-regulated on peripheral blood T cells from OLP patients and B7-H1 expression positively correlated with disease severity of OLP. It is suggested that Th1 dominant inflammatory situation might contribute to the high expression of PD-1 and B7-H1 in OLP. Blockade of PD-1/B7-H1 pathway significantly increased the proliferation, and IFN-γ and IL-2 production of T cells.

Conclusions

PD-1/B7-H1 pathway may play an important role in negatively modulating T cell-mediated immune response in OLP, and provide the rationale to employ B7-H1 expression on peripheral blood T cells as a marker of severity of OLP and to develop agonists targeting PD-1/B7-H1 pathway as a promising immunotherapeutic strategy for OLP.

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Acknowledgments

This work was supported by a Grant (No. 30973311, No. 81170972) from National Natural Science Foundation of China.

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Authors and Affiliations

  1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China

    Gang Zhou, Jing Zhang, Xiang-wei Ren, Jing-yu Hu & Ge-fei Du

  2. Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Luoyu Road 237, Wuhan, People’s Republic of China, 430079

    Gang Zhou, Ge-fei Du & Xue-yi Xu

Authors
  1. Gang Zhou
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  2. Jing Zhang
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  3. Xiang-wei Ren
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  4. Jing-yu Hu
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  5. Ge-fei Du
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  6. Xue-yi Xu
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Corresponding author

Correspondence to Gang Zhou.

Additional information

Gang Zhou and Jing Zhang contributed equally to this work.

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Zhou, G., Zhang, J., Ren, Xw. et al. Increased B7-H1 Expression on Peripheral Blood T Cells in Oral Lichen Planus Correlated with Disease Severity. J Clin Immunol 32, 794–801 (2012). https://doi.org/10.1007/s10875-012-9683-2

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  • DOI: https://doi.org/10.1007/s10875-012-9683-2

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