Abstract
Purpose
Children with down syndrome (DS) have an increased susceptibility to infections, due to altered humoral and/or cellular immunity. The aim of this study was to determine the cytokine production in whole blood of children with DS upon stimulation with live influenza A virus.
Methods
Whole blood of 61 children with DS and 57 of their healthy siblings was stimulated with 2.5 × 104 TCID50/ml influenza A virus during 6, 24, and 48 h. TNF-α, IL-1β, IL-6, IL-8, IL-10, IL-12p70, IFN-α, IFN-γ concentrations, and viral load were measured at all time points.
Results
At most of the time points, TNF-α, IL-1β, IL-6, and IL-8 concentrations were significantly higher in children with DS following stimulation with live influenza A virus. IFN-α and IFN-γ levels were also significantly higher in the DS group. Viral clearance, however, was equal in both groups.
Conclusions
Children with DS have an altered immune response to influenza A virus. The production of higher levels of pro-inflammatory cytokines may be responsible for a more severe clinical course of viral disease in these children.
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Abbreviations
- DS:
-
Down syndrome
- RTI:
-
Respiratory tract infection
- RSV:
-
Respiratory syncytial virus
- TCID50 :
-
Median tissue culture infective dose
- PBS:
-
Phosphate-buffered saline
- SEM:
-
Standard error of the mean
- TLR:
-
Toll-like receptor
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Acknowledgments
The authors thank Jacqueline Cloos, Petra van den Pangaart, and Eva Brandse for their excellent advice and technical support.
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The authors declare that they have no conflict of interest.
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The study has been approved by the appropriate ethics committee and have been performed in accordance with the 1964 Declaration of Helsinki and its later amendments.
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Broers, C.J.M., Gemke, R.J.B.J., Weijerman, M.E. et al. Increased Pro-inflammatory Cytokine Production in Down syndrome Children Upon Stimulation with Live Influenza A Virus. J Clin Immunol 32, 323–329 (2012). https://doi.org/10.1007/s10875-011-9625-4
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DOI: https://doi.org/10.1007/s10875-011-9625-4