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Lack of Association between ORAI1/CRACM1 Gene Polymorphisms and Kawasaki Disease in the Taiwanese Children

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Abstract

Objective

Kawasaki disease (KD) is characterized by systemic vasculitis of an unknown cause. A previous study has indicated that a polymorphism of the inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) gene is involved in the susceptibility to KD. ORAI (also known as CRACM1) is one of the components of store-operated calcium channels involved in regulating immune and inflammatory reactions. This study was conducted to investigate if polymorphisms in ORAI1/CRACM1, a gene downstream from ITPKC, are associated with KD susceptibility and clinical outcomes.

Materials and Methods

A total of 1,056 subjects (341 KD patients and 715 controls) were investigated to identify five tagging single nucleotide polymorphisms (tSNPs) in ORAI1/CRACM1 (rs12313273, rs6486795, rs7135617, rs12320939, and rs712853) by using the TaqMan Allelic Discrimination assay.

Results

No significant associations between genotype and allele frequency of the five ORAI1/CRACM1 tSNPs were observed in the KD patients and controls. In KD patients, no significant associations between ORAI1/CRACM1 polymorphisms and coronary artery lesion (CAL) formation or intravenous immunoglobulin (IVIG) treatment response were observed. The results from haplotype analysis were insignificant.

Conclusions

This study showed for the first time that ORAI1/CRACM1 polymorphisms are not associated with KD susceptibility, CAL formation, or IVIG treatment response in the Taiwanese population.

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Abbreviations

KD:

Kawasaki disease

IVIG:

Intravenous immunoglobulin

CAL:

Coronary artery lesions

CRACM1 :

Calcium release-activated calcium (CRAC) modulator 1

ITPKC :

Inositol 1,4,5-trisphosphate 3-kinase C

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Acknowledgements

I am grateful to Dr. Yoshihiro Onouchi (RIKEN) for reading this manuscript. This study was partly supported by funding from Excellence for Cancer Research Center grant, Department of Health, Executive Yuan, Taiwan, ROC (NO. DOH100-TD-C-111-002); grant from the National Science Council, Taiwan, ROC (NSC 98-2320-B-037-028-MY2 and NSC97-2314-B-182A-054-MY2); grant from the Center of Excellence for Environmental Medicine, Kaohsiung Medical University (KMU-EM-99-6-3); and grant from Chang Gung Memorial Hospital, Taiwan, ROC (CMRPG891441 and CMRPG891241).

Competing interests

The authors declare no competing interests.

Author information

Authors and Affiliations

  1. Division of Allergy, Immunology and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital–Kaohsiung Medical Center, Kaohsiung, Taiwan

    Ho-Chang Kuo, Kuender D. Yang, Hong-Ren Yu & Li-Yan Lin

  2. Division of Cardiology, Department of Pediatrics, Chang Gung Memorial Hospital–Kaohsiung Medical Center, Kaohsiung, Taiwan

    Ying-Jui Lin & Chi-Di Liang

  3. Graduate Institute of Clinical Medical Science, Chang Gung University College of Medicine, Kaohsiung, Taiwan

    Ho-Chang Kuo, Kuender D. Yang & Hong-Ren Yu

  4. Department of Medical Genetics, College of Medicine, Kaohsiung Medical University, 100 TzYou First Road, Kaohsiung City, 807, Taiwan

    Suh-Hang Hank Juo, Yu-Wen Hsu, Wei-Chiao Chen, Mei-Chyn Chao & Wei-Chiao Chang

  5. Center of Excellence for Environmental Medicine, Kaohsiung Medical University, 100 TzYou First Road, Kaohsiung City, 807, Taiwan

    Wei-Chiao Chang

  6. Center of Excellence for Environmental Medicine, Kaohsiung Medical University, 100 TzYou First Road, Kaohsiung City, 807, Taiwan

    Suh-Hang Hank Juo & Wei-Chiao Chang

  7. Comprehensive Melanoma Research Center and Department of Tumor Biology, H. Lee Moffitt Cancer Center, Tampa, FL, USA

    Shengyu Yang

  8. Institute of Sound and Vibration Research, University of Southampton, Southampton, UK

    Shouyan Wang

  9. Division of Genetics, Endocrinology and Metabolism, Department of Pediatrics, Kaohsiung Medical University Hospital, 100 TzYou First Road, Kaohsiung City, 807, Taiwan

    Mei-Chyn Chao

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  1. Ho-Chang Kuo
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  2. Ying-Jui Lin
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  13. Wei-Chiao Chang
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Corresponding author

Correspondence to Wei-Chiao Chang.

Additional information

Ho-Chang Kuo and Ying-Jui Lin contributed equally to this article.

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Kuo, HC., Lin, YJ., Juo, SH.H. et al. Lack of Association between ORAI1/CRACM1 Gene Polymorphisms and Kawasaki Disease in the Taiwanese Children. J Clin Immunol 31, 650–655 (2011). https://doi.org/10.1007/s10875-011-9524-8

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  • DOI: https://doi.org/10.1007/s10875-011-9524-8

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