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Investigation of Functional IL-10 Gene Polymorphism and IL-10 Levels in Acute Graft-Versus-Host Disease

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Abstract

Background

Graft-versus-host disease (GVHD) represents a major complication in allogeneic hematopoietic stem cell transplant (allo-HSCT) recipients. Although studies have been conducted concerning the investigation of cytokine polymorphisms in the development of acute GVHD (aGVHD), the contribution of recipients and donors as regards cytokine levels has not yet been thoroughly assessed.

Objective

The aim of this study was to investigate the impact of IL-10 polymorphisms on cytokine levels in blood and saliva, in addition to the occurrence and severity of aGVHD.

Methods

Fifty-eight consecutive allo-HSCT recipients and their donors were included in this prospective study. Saliva and/or blood samples were obtained from recipients and donors to determine IL10 polymorphisms. The IL-10 levels in the blood and saliva were also assessed. The samples were collected from seven days before transplant (day −7) to 100 days after allo-HSCT (day +100), once a week or until the death of recipient.

Results

No association was found between recipient and donor IL10 polymorphism and IL-10 levels in the saliva with aGVHD. In contrast, IL-10 levels in the blood were associated with the occurrence of aGVHD. The high producer phenotype in the recipient was also associated with high levels of IL-10 in the blood and saliva.

Conclusion

Although IL10 polymorphisms were not associated with the occurrence and severity of aGVHD, the genetic background of the recipient did in fact influence the production of the cytokine. Furthermore, as IL-10 levels in the blood were associated with the disease development, this parameter may well be a useful predictor of aGVHD development.

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Acknowledgements

This study was supported in part by grants from Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil. RS Gomez and TA Silva are research fellows of CNPq. We thank Daniele Ramalho da Silva for technical assistance.

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Authors and Affiliations

  1. Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Renata Gonçalves Resende, Jeane de Fátima Correia-Silva, Telma Cristina Arão, Tarcília Aparecida Silva & Ricardo Santiago Gomez

  2. Department of Preventive Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Mauro Henrique Nogueira Guimarães Abreu

  3. Stem Cell Transplant Unit, Hospital das Clinicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Henrique Bittencourt

  4. Faculdade de Odontologia, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627, Belo Horizonte, Minas Gerais, Cep 31270-901, Brazil

    Ricardo Santiago Gomez

Authors
  1. Renata Gonçalves Resende
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  2. Jeane de Fátima Correia-Silva
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  3. Telma Cristina Arão
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  4. Tarcília Aparecida Silva
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  5. Mauro Henrique Nogueira Guimarães Abreu
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  6. Henrique Bittencourt
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  7. Ricardo Santiago Gomez
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Correspondence to Ricardo Santiago Gomez.

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Resende, R.G., Correia-Silva, J.d.F., Arão, T.C. et al. Investigation of Functional IL-10 Gene Polymorphism and IL-10 Levels in Acute Graft-Versus-Host Disease. J Clin Immunol 30, 465–473 (2010). https://doi.org/10.1007/s10875-010-9377-6

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  • DOI: https://doi.org/10.1007/s10875-010-9377-6

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