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Postmenopausal Expression Changes of Immune System-Related Genes in Human Bone Tissue

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Abstract

Introduction

The molecular and cellular interactions between the immune system and bone tissue have been established. Sex hormone deficiency after menopause has multifunctional role by influencing growth, differentiation, and metabolism of the skeletal and the immune system.

Discussion

We have used nonparametric and multidimensional expression pattern analyses to determine significantly changed mRNA profile of immune system-associated genes in postmenopausal (POST) and premenopausal (PRE) nonosteoporotic bone. Ten bone tissue samples from POST patients and six bone tissue samples from PRE women were examined in our study. The transcription differences of the selected 50 genes were analyzed in TaqMan probe-based quantitative real-time reverse transcriptase polymerase chain reaction system. Mann–Whitney test indicated significantly downregulated transcription activity of three genes (CD14, HLA-A/MHCI, ITGAM/CD11b) and upregulated expression of six genes (C3, CD86/B7-2, IL-10, IL-6, TGFB3, TNFSF11/RANKL) in postmenopausal bone. According to the canonical variate analysis results, the groups of POST and PRE women are separable by genes coding for cytokines, costimulator molecules, and cell surface receptors involved in antigen presentation and T cell stimulation processes which have high discriminatory power. Based on a complex gene expression pattern analysis of human bone tissue, we could distinguish POST and PRE states from an immunological aspect. Our data might provide further insight into the changes of the intersystem crosstalk between immune and skeletal homeostasis, as well as local immune response in the altered microenvironment of postmenopausal bone.

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Acknowledgments

This work was supported by grants NKFP-1A/007/2004 and NKFP-1A/002/2004 from the National Research and Technological Office (NKTH) of Hungary, as well as by research grant ETT 022/2006 from the Ministry of Health, Hungary. J. Podani was supported by a Hungarian Scientific Research Fund (OTKA) grant no. NI 68218. J. Kiss was supported by OTKA grant no. T-037436.

Conflict of interest

All the authors hereby state that they do not possess financial interests and they have no conflicts of interest.

Author information

Authors and Affiliations

  1. 1st Department of Internal Medicine, Semmelweis University, Koranyi 2/a, Budapest, 1083, Hungary

    János P. Kósa, Bernadett Balla, István Takács, Áron Lazáry, Zsolt Nagy, Krisztián Bácsi, Gábor Speer & Péter Lakatos

  2. Department of Orthopaedics, Semmelweis University, Budapest, Hungary

    János Kiss

  3. Department of Plant Taxonomy and Ecology, Eötvös Loránd University, Budapest, Hungary

    János Podani

  4. Department of Microbiology, Eötvös Loránd University, Budapest, Hungary

    Attila Karsai

Authors
  1. János P. Kósa
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  2. Bernadett Balla
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  3. János Kiss
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  4. János Podani
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  5. István Takács
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  7. Zsolt Nagy
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  8. Krisztián Bácsi
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  9. Attila Karsai
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  10. Gábor Speer
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  11. Péter Lakatos
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Corresponding author

Correspondence to János P. Kósa.

Additional information

J. P. Kósa and B. Balla equally contributed to this work.

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Kósa, J.P., Balla, B., Kiss, J. et al. Postmenopausal Expression Changes of Immune System-Related Genes in Human Bone Tissue. J Clin Immunol 29, 761–768 (2009). https://doi.org/10.1007/s10875-009-9321-9

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  • DOI: https://doi.org/10.1007/s10875-009-9321-9

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