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Tissue Transglutaminase Can Be Involved in Airway Inflammation of Toluene Diisocyanate-Induced Occupational Asthma

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Abstract

Background

This study was conducted to evaluate whether tissue transglutaminase (tTG) may be involved in airway inflammation of toluene diisocyanate-induced occupational asthma (TDI-OA).

Methods

We enrolled 93 patients with TDI-OA, 177 asymptomatic exposed subjects, 43 patients with allergic asthma, and 70 unexposed normal controls. The prevalence of serum immunoglobulin G (IgG) to tTG in the TDI-OA group (20.2%) was significantly higher than that in the three other groups (P < 0.001).

Results

TDI-OA patients with serum IgG to tTG had significantly lower methacholine PC20 values (P < 0.02) and significantly higher prevalence of specific immunoglobulin E to vapor type TDI–human serum albumin conjugate (P < 0.01; r 2 = 0.411, P < 0.05). TDI exposure could increase tTG activity via reactive oxygen species (ROS) production, which was found to cross-link with cytokeratin 19 on immunoblot analysis.

Conclusion

Therefore, TDI exposure may activate tTG via ROS-mediated mechanism in the airway epithelium leading to persistent airway inflammation in TDI-OA patients.

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Acknowledgments

This study was supported by a grant from the Korean Health 12 R&D project, Ministry of Health & Welfare, Republic of Korea (A030001) and Daewoo Medical Foundation, 2008.

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Authors and Affiliations

  1. Department of Internal Medicine, Korea University College of Medicine, Seoul, South Korea

    Gyu-Young Hur

  2. Department of Allergy and Rheumatology, Ajou University School of Medicine, San-5, Wonchon-dong, Youngtong-gu, Suwon, 443-721, South Korea

    Sung-Ho Kim, Young-Min Ye & Hae-Sim Park

  3. Pharmacology and Chronic Inflammatory Disease Research Center, Ajou University School of Medicine, Suwon, South Korea

    Sang Myun Park

  4. Department of Internal Medicine, Inha University College of Medicine, Incheon, South Korea

    Cheol-Woo Kim

  5. Department of Internal Medicine, Soonchunhyang University Hospital, Bucheon, South Korea

    An-Soo Jang & Choon-Sik Park

  6. Department of Internal Medicine, Yonsei University School of Medicine, Seoul, South Korea

    Chein Soo Hong

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  1. Gyu-Young Hur
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Correspondence to Hae-Sim Park.

Additional information

Gyu-Young Hur and Sung-Ho Kim equally contributed to this work.

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Hur, GY., Kim, SH., Park, S.M. et al. Tissue Transglutaminase Can Be Involved in Airway Inflammation of Toluene Diisocyanate-Induced Occupational Asthma. J Clin Immunol 29, 786–794 (2009). https://doi.org/10.1007/s10875-009-9314-8

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  • DOI: https://doi.org/10.1007/s10875-009-9314-8

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