Abstract
Introduction
Cytokines play a significant role in host immune defense. IL-10 is an anti-inflammatory, immunomodulatory cytokine that can both stimulate and suppress the immune response and inhibits HIV-1 replication in vivo. Interindividual variations in IL-10 production were genetically contributed to polymorphisms within IL-10 promoter region.
Aims
The aim of this study was to investigate the association of IL-10 gene promoter −1082 G/A, −819 C/T, and 592 C/A polymorphism on HIV-1 transmission /progression in North Indian individuals.
Patients and Methods
A total of 180 HIV-1 seropositive (HSP) stratified on the basis of disease severity (stage I, II, and III), 50 HIV-1 exposed seronegative (HES) and 305 HIV-1 seronegative (HSN) individuals were genotyped for IL-10 gene promoter by polymerase chain reaction-restriction fragment length polymorphism. A suggestive evidence of association was obtained for IL-10 592 C/A promoter polymorphism at the level of allele and genotype distribution. The frequency of IL-10 592 A allele and genotype was significantly increased in HSP compared to HSN (p = 0.013; OR = 1.412 and p = 0.034; OR = 1.685 respectively). Further comparison in between different clinical stages of HIV-1 infected patients of IL-10 592 A allele and genotype revealed a significant increase in its frequency in the stage III compared with those together in stage I (p = 0.004, OR = 2.181 and p = 0.002, OR = 4.156, respectively). This study reports for the first time that IL-10 gene promoter 592 C/A polymorphism may be a risk factor for HIV-1 transmission/progression in HIV-1 infected North Indian individuals.
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Acknowledgement
We thank Mr. Pradeep K. Singhal, Professional Biotech Pvt. Ltd., New Delhi for unwavering support and for insightful advice. We gratefully thank Mr. S. Pandey, V. K. Mishra, Department of Microbiology, SGPGIMS, Lucknow for assistance in coordinating the clinical samples and all of the individuals who participated in the study.
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Chatterjee, A., Rathore, A., Sivarama, P. et al. Genetic Association of IL-10 Gene Promoter Polymorphism and HIV-1 Infection in North Indians. J Clin Immunol 29, 71–77 (2009). https://doi.org/10.1007/s10875-008-9220-5
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DOI: https://doi.org/10.1007/s10875-008-9220-5