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Bystander Effect in Synergy to Anergy in Oral Tolerance of Blomia Tropicalis/Ovalbumin Murine Co-Immunization Model

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Abstract

Oral tolerance is an important approach in allergic diseases and murine model can provide useful information to improve its understanding and therapeutic measures. To address the influence of non-related allergen sensitization in immunized mice with the mite Blomia tropicalis (Bt) or ovalbumin (OVA) or with both Bt/OVA allergens. Furthermore, we sought to verify oral tolerance effect in the Bt/OVA co-immunization model. Mice sensitized with Bt and then exposed to OVA developed an enhanced IgE response to both allergens; contrariwise, this effect was not observed when OVA-sensitization was prior to Bt-sensitization. Co-injection of Bt and OVA led to a dominant IgE response towards OVA over Bt, which was not observed when co-immunization was performed with a 240-fold less amount of OVA. Induction of oral tolerance with OVA, prior to co-immunization, suppressed IgE response to both allergens, probably as a consequence of the increased levels of IFN-γ found in these animals. The results evidenced that, depending on allergenic potential, new allergen exposure may exert an adjuvant effect to the first allergen used in the sensitization. The bystander suppression to non-related allergens through oral tolerance should be a useful mechanism to control sensitization to new allergens.

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Authors and Affiliations

  1. Laboratory of Clinical and Experimental Allergy and Immunology, School of Medicine, University of São Paulo, LIM 56, Brazil

    C. R. Oliveira, E. A. F. Taniguchi, A. E. Fusaro, J. R. Victor, C. A. Brito, A. J. S. Duarte & M. N. Sato

  2. Laboratório de Alergia e Imunologia Clínica e Experimental, Faculdade de Medicina da Universidade de São Paulo, Instituto de Medicina Tropical—Prédio II, Av Dr Enéas de Carvalho Aguiar, 500, 3° andar, 05403-000, São Paulo, Brazil

    M. N. Sato

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  1. C. R. Oliveira
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  2. E. A. F. Taniguchi
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  3. A. E. Fusaro
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  4. J. R. Victor
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  5. C. A. Brito
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  6. A. J. S. Duarte
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  7. M. N. Sato
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Correspondence to M. N. Sato.

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Oliveira, C.R., Taniguchi, E.A.F., Fusaro, A.E. et al. Bystander Effect in Synergy to Anergy in Oral Tolerance of Blomia Tropicalis/Ovalbumin Murine Co-Immunization Model. J Clin Immunol 25, 153–161 (2005). https://doi.org/10.1007/s10875-005-2821-3

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  • DOI: https://doi.org/10.1007/s10875-005-2821-3

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