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Oxidative Stress Level in Circulating Neutrophils Is Linked to Neurodegenerative Diseases

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Abstract

Alzheimer’s and Parkinson’s diseases are the most common neurodegenerative conditions. Oxidative lesions are a hallmark of both diseases, but the respective roles of systemic and cerebral dysfunction are not elucidated. As circulating neutrophils are the most powerful sources of reactive oxygen species, we measured oxidative stress levels in resting neutrophils from 44 Alzheimer’s and Parkinson’s disease patients and compared them to 40 healthy counterparts. Significantly increased oxidative stress levels were observed in patients’ groups, while control groups had very similar levels irrespective of age. One-third of the neurodegenerative patients presented with oxidative stress levels higher than those of any healthy donor. This increase was not due to an elevated production of reactive oxygen species during the neutrophil oxidative burst. Mitochondrial mass and activity were altered in neutrophils of the Parkinsonian group compared to controls, but not in those from Alzheimer’s disease group. To our knowledge, this is the first report linking oxidative stress and mitochondrial parameters in circulating neutrophils from neurodegenerative and normal donors. Our results indicate that oxidative stress levels in circulating neutrophils are of interest for further mechanistic studies of neurodegenerative diseases and might open the perspective of a diagnostic tool.

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Authors and Affiliations

  1. Laboratoire d’Immunologie, INSERM UMR 600/CNRS FRE 2059, APHM Hôpital Sainte-Marguerite, 270, boulevard de Sainte-Marguerite, 13274, Marseille Cedex 09, France

    JOANA VITTE & PIERRE BONGRAND

  2. Fédération de Neuro-Géronto-Psychiatrie, APHM Hôpital Sainte-Marguerite, 270, boulevard de Sainte-Marguerite, 13274, Marseille Cedex 09, France

    BERNARD F. MICHEL

  3. Centre d’Investigation Clinique 9502, APHM Hôpital Sainte-Marguerite, 270 boulevard de Sainte-Marguerite, 13274, Marseille Cedex 09, France

    BERNARD F. MICHEL & PIERRE BONGRAND

  4. Service de Neurologie, APHM Hôpital Sainte-Marguerite, 270, boulevard de Sainte-Marguerite, 13274, Marseille Cedex 09, France

    JEAN-LOUIS GASTAUT

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  1. JOANA VITTE
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  2. BERNARD F. MICHEL
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  4. JEAN-LOUIS GASTAUT
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VITTE, J., MICHEL, B.F., BONGRAND, P. et al. Oxidative Stress Level in Circulating Neutrophils Is Linked to Neurodegenerative Diseases. J Clin Immunol 24, 683–692 (2004). https://doi.org/10.1007/s10875-004-6243-4

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  • DOI: https://doi.org/10.1007/s10875-004-6243-4

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