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Imaging and determining friction forces of specific interactions between human IgG and rat anti-human IgG

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Abstract

Covalently immobilized rat anti-human immunoglobulin (IgG) monolayers on thiol-modified gold substrates and human IgG linked with the tips were fabricated using the self-assembled monolayer method, and interactions between these systems were studied by friction force microscopy (FFM). In addition to observation of distinct nanostructures of protein monolayers due to recognition events, FFM also quantified the friction force due to protein–protein-specific interactions. The average friction force due to interactions between the antigen functionalized tip and the antibody monolayer was determined as 200–250 pN, significantly greater than that between either the bare tip and the antibody monolayer (0–50 pN), or the blocked antigen tip and the antibody monolayer (50–100 pN), indicative of antigen/antibody-specific interactions. These results, taken together, suggest that FFM is not only capable of tracking recognition events, but also quantifying the friction force due to specific interactions between biological molecules, such as antigen and antibody.

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Acknowledgements

This work was supported by Grants from the Natural Science Foundation of China (No. 30670496 to JHW, No. 30770529 to LHD), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Ministry of Education (No. 2006-331 to JHW) and the Natural Science Foundation of Chongqing-Commission of Science and Technology (CSTC) (No. 2006BB5017 to JHW, No. 2010BA5001 to LHD). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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  1. Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Institute of Biochemistry and Biophysics, College of Bioengineering, Chongqing University, Chongqing, 400044, China

    Zhengjian Lv, Jianhua Wang, Guoping Chen & Linhong Deng

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  1. Zhengjian Lv
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  2. Jianhua Wang
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  3. Guoping Chen
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Correspondence to Jianhua Wang or Linhong Deng.

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Lv, Z., Wang, J., Chen, G. et al. Imaging and determining friction forces of specific interactions between human IgG and rat anti-human IgG. J Biol Phys 37, 417–427 (2011). https://doi.org/10.1007/s10867-011-9223-y

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