Abstract
We studied the dynamics of the Heat Shock Response (HSR) mechanism, and the persistence of a injury-protected state in the cell following the shocks, known as thermotolerance. A series of double shock experiments were performed on Chinese Hamster Ovary (CHO) cells, tracking the dynamics of some components of HSR pathway (the Hsp70 protein level and Hsp70 mRNA transcription rate). The main features of HSR dynamics were well reproduced by a simplified model of the chemical reaction pathways governing the HSR. In particular, the thermotolerance phenomenon could be well characterized by introducing a shock-dependent switch in mRNA halflife, that can be interpreted as a sort of primitive memory at the mRNA level.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ritossa, F.: New Puffing Pattern Induced by Temperature Shock and DNP in Drosophila, Experientia 18 (1962), 571–573.
Tissieres, A., Mitchell, H.K. and Tracy, U.M.: Protein Synthesis in Salivary Glands of D. melanogaster: Relation to Chromosome Puffs, J. Mol. Biol. 84 (1974), 389–398.
Morimoto, R.I.: Regulation of the Heat Shock Transcriptional Response: Cross Talk Between a Family of Heat Shock Factors, Molecular Chaperones, and Negative Regulators, Genes Dev. 12 (1998), 3788.
Kiang, J.G. and Tsokos, G.C.: Heat Shock Protein 70 kDa: Molecular Biology, Biochemistry, and Physiology, Pharmacol. Ther. 80(2) (1998), 183–201.
de Pomerai, D., Daniells, C., David, H., Allan, J., Duce, I., Mutwakil, M., Thomas, D., Sewell, P., Tattersall, J., Jones, D., Candido, P.: Non-Thermal Heat-Shock Response to Microwaves, Nature 405 (2000), 417.
Arnvig, K.B., Pedersen, S. and Sneppen, K.: Thermodynamics of Heat Shock Response. (cond- mat/9912402), Phys. Rev. Lett. 84:13 (2000), 3005.
Theodorakis, N.G., Drujan, D., De Maio, A.: Thermotolerant Cells Show an Attenuated Expression of Hsp70 After Heat Shock, J. Biol. Chem. 274:17 (1999), 12081.
Bonner, J.J., Carlson, T., Fackenthal, D.L., Paddock, D., Storey, K., Lea, K.: Complex Regulation of the Yeast Heat Shock Transcription Factor, Mol. Biol. Cell 11(5) (2000), 1739–1751.
Morimoto, R.I., Tissières, A. and Georgopoulos, C. (eds.): The Biology of Heat Shock Proteins and Molecular Chaperones, Cold Spring Harbor Laboratory Press, 1994.
Kiang, J.G., Tsokos, G.C.: Heat Shock Protein 70 kDa: Molecular Biology, Biochemistry, and Physiology, Pharmacol. Ther. 80(2) (1998), 183.
Theodorakis, N.G. and Morimoto, R.I.: Posttranscriptional Regulation of Hsp70 Expression in Human Cells: Effects of Heat Shock, Inhibition of Protein Synthesis, and Adenovirus Infection on Translation and mRNA Stability, Mol. Cell. Biol. 7(12) (1987), 4357.
Li, D. et al.: Eur. J. Biochem. 231(2) (1995), 454.
Barbieri, M.: The Organic Codes, Cambridge Univ. Press, Cambridge, 2003.
Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K. and Watson, J.: Molecular Biology of the Cell III ed., Garland, New York, 1994.
Hartl, F.U. and Hayer-Hartl, M.: Molecular Chaperones in the Cytosol: From Nascent Chain to Folded Protein, Science 295 (2002), 1852–1858, DOI: 10.1126/science.1068408
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Remondini, D., Bernardini, C., Forni, M. et al. Induced Metastable Memory in Heat Shock Response. J Biol Phys 32, 49–59 (2006). https://doi.org/10.1007/s10867-006-2670-1
Issue Date:
DOI: https://doi.org/10.1007/s10867-006-2670-1