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The cytochrome c oxidase and its mitochondrial function in the whiteleg shrimp Litopenaeus vannamei during hypoxia

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Abstract

Cytochrome c oxidase (COX), which is located in the inner membrane of mitochondria, is a key constituent of the electron transport chain that catalyzes the reduction of oxygen. The Pacific whiteleg shrimp Litopenaeus vannamei is constantly exposed to hypoxic conditions, which affects both the central metabolism and the mitochondrial function. The purpose of this study was to isolate shrimp mitochondria, identify the COX complex and to evaluate the effect of hypoxia on the shrimp mitochondrial function and in the COX activity. A 190 kDa protein was identified as COX by immunodetection techniques. The effect of hypoxia was confirmed by an increase in the shrimp plasma L-lactate concentration. COX activity, mitochondrial oxygen uptake and protein content were reduced under hypoxic conditions, and gradually restored as hypoxia continued, this suggests an adaptive mitochondrial response and a highly effective COX enzyme. Both mitochondrial oxygen uptake and COX activity were completely inhibited by KCN and sodium azide, suggesting that COX is the unique oxidase in L. vannamei mitochondria.

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Authors and Affiliations

  1. Laboratory of Bioenergetics and Molecular Genetics, Centro de Investigacion en Alimentacion y Desarrollo, A.C., Carretera a Ejido La Victoria Km 0.6, PO Box. 1735, Hermosillo, Sonora, 83000, Mexico

    L. R. Jimenez-Gutierrez, C. Chimeo & A. Muhlia-Almazan

  2. Department of Molecular Genetics, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, P.O. Box, 70-242, Mexico, D.F., 04510, Mexico

    S. Uribe-Carvajal

  3. Laboratorio de Referencia, Analisis y Diagnostico en Sanidad Acuicola, Centro de Investigaciones Biologicas del Noroeste (CIBNOR), S.C. (Campus Hermosillo), Calle Hermosa No. 101. Col. Los Angeles, Hermosillo, Sonora, 83106, Mexico

    A. Sanchez-Paz

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  1. L. R. Jimenez-Gutierrez
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  2. S. Uribe-Carvajal
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  3. A. Sanchez-Paz
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  4. C. Chimeo
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  5. A. Muhlia-Almazan
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Correspondence to A. Muhlia-Almazan.

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Jimenez-Gutierrez, L.R., Uribe-Carvajal, S., Sanchez-Paz, A. et al. The cytochrome c oxidase and its mitochondrial function in the whiteleg shrimp Litopenaeus vannamei during hypoxia. J Bioenerg Biomembr 46, 189–196 (2014). https://doi.org/10.1007/s10863-013-9537-5

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  • DOI: https://doi.org/10.1007/s10863-013-9537-5

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