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The basidiomycete Ustilago maydis has two plasma membrane H+-ATPases related to fungi and plants

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Abstract

The fungal and plant plasma membrane H+-ATPases play critical roles in the physiology of yeast, plant and protozoa cells. We identified two genes encoding two plasma membrane H+-ATPases in the basidiomycete Ustilago maydis, one protein with higher identity to fungal (um02581) and the other to plant (um01205) H+-ATPases. Proton pumping activity was 5-fold higher when cells were grown in minimal medium with ethanol compared to cells cultured in rich YPD medium, but total vanadate-sensitive ATPase activity was the same in both conditions. In contrast, the activity in cells cultured in minimal medium with glucose was 2-fold higher than in YPD or ethanol, implicating mechanisms for the regulation of the plasma membrane ATPase activity in U. maydis. Analysis of gene expression of the H+-ATPases from cells grown under different conditions, showed that the transcript expression of um01205 (plant-type) was higher than that of um02581 (fungal-type). The translation of the two proteins was confirmed by mass spectrometry analysis. Unlike baker’s yeast and plant H+-ATPases, where the activity is increased by a short incubation with glucose or sucrose, respectively, U. maydis H+-ATPase activity did not change in response to these sugars. Sequence analysis of the two U. maydis H+-ATPases revealed the lack of canonical threonine and serine residues which are targets of protein kinases in Saccharomyces cerevisiae and Arabidopsis thaliana plasma membrane H+-ATPases, suggesting that phosphorylation of the U. maydis enzymes occurs at different amino acid residues.

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  1. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala S/N, Miguel Hidalgo, 11340, México, D.F., México

    Leobarda Robles-Martínez & Guadalupe Guerra-Sánchez

  2. Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70-159, Coyoacán, 04510, México, D.F., México

    Juan Pablo Pardo, Macario Genaro Matus-Ortega & Guillermo Mendoza-Hernández

  3. Department of Biological Sciences, University of Texas at El Paso, 500 West University Avenue, El Paso, TX, 79968, USA

    Manuel Miranda & Tavis L. Mendez

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  1. Leobarda Robles-Martínez
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  2. Juan Pablo Pardo
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  3. Manuel Miranda
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  4. Tavis L. Mendez
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Correspondence to Juan Pablo Pardo or Guadalupe Guerra-Sánchez.

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Juan Pablo Pardo and Guadalupe Guerra-Sánchez contributed equally to this work.

Guillermo Mendoza-Hernández in memory to his contribution.

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Robles-Martínez, L., Pardo, J.P., Miranda, M. et al. The basidiomycete Ustilago maydis has two plasma membrane H+-ATPases related to fungi and plants. J Bioenerg Biomembr 45, 477–490 (2013). https://doi.org/10.1007/s10863-013-9520-1

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