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Mitochondrial haplotype divergences affect specific temperature sensitivity of mitochondrial respiration

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Abstract

The aim of this study was to investigate the effect of temperature changes on the functional properties of mitochondria from two sets of D. simulans fly lines harboring the siII and siIII haplotypes in a common nuclear genetic background. We studied four introgressed isofemale lines possessing the mtDNA of siII and the nuclear background of siIII (siII-introgressed) and four lines possessing siIII mitochondria with its native nuclear genome (siIII-controls). We assessed the catalytic capacities of electron transport system (ETS) at four different temperatures (12, 18, 24 and 28 ºC). The impact of temperature on the pyruvate dehydrogenase (PDH) activity, the mitochondrial respiration (coupled and uncoupled respiration), cytochrome c oxidase activity, as well as the excess capacity of complex IV (COX) were evaluated in these two sets of flies. Our results showed that the temperature coefficient values (Q10) measured for mitochondrial respiration in the lower range of temperatures (12 to 18 ºC) showed a 2 to 3 fold increase in siII-introgressed when compared to siIII-controls. This result shows that the impact of temperature on mitochondrial function is different between the two mitotypes studied. The Q10 results seem to be linked to the apparent COX excess capacity of 193 % for siIII-controls that is inexistent for siII-introgressed at 12 ºC. One explanation for these results is that the mitochondria can compensate for the disruption of mito-nuclear interactions at 24 ºC but not at lower temperatures. An alternate explanation would be that siII haplotype confer divergent kinetic properties to the ETS that translate to different temperature sensitivities.

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Authors and Affiliations

  1. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia

    Nicolas Pichaud & J. William O. Ballard

  2. Laboratoire de Génétique Cellulaire et Développementale, Département de Biologie Moléculaire, Biochimie Médicale et Pathologie, Institut de Biologie Intégrative et des Systèmes, Université Laval, 1030 ave de la Médecine, Québec, Canada, G1V 0A6

    Robert M. Tanguay

  3. Laboratoire de Biologie Intégrative, Département de Biologie, Université du Québec à Rimouski, 300 allée des Ursulines, Rimouski, Québec, Canada, G5L 3A1

    Pierre U. Blier

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  1. Nicolas Pichaud
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  2. J. William O. Ballard
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  4. Pierre U. Blier
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Correspondence to Pierre U. Blier.

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Pichaud, N., Ballard, J.W.O., Tanguay, R.M. et al. Mitochondrial haplotype divergences affect specific temperature sensitivity of mitochondrial respiration. J Bioenerg Biomembr 45, 25–35 (2013). https://doi.org/10.1007/s10863-012-9473-9

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