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Absolute levels of transcripts for mitochondrial uncoupling proteins UCP2, UCP3, UCP4, and UCP5 show different patterns in rat and mice tissues

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Abstract

Existing controversies led us to analyze absolute mRNA levels of mitochondrial uncoupling proteins (UCP1-UCP5). Individual UCP isoform mRNA levels varied by up to four orders of magnitude in rat and mouse tissues. UCP2 mRNA content was relatively high (0.4 to 0.8 pg per 10 ng of total mRNA) in rat spleen, rat and mouse lung, and rat heart. Levels of the same order of magnitude were found for UCP3 mRNA in rat and mouse skeletal muscle, for UCP4 and UCP5 mRNA in mouse brain, and for UCP2 and UCP5 mRNA in mouse white adipose tissue. Significant differences in pattern were found for rat vs. mouse tissues, such as the dominance of UCP3/UCP5 vs. UCP2 transcript in mouse heart and vice versa in rat heart; or UCP2 (UCP5) dominance in rat brain contrary to 10-fold higher UCP4 and UCP5 dominance in mouse brain. We predict high antioxidant/antiapoptotic UCP function in tissues with higher UCP mRNA content.

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Authors and Affiliations

  1. Department of Membrane Transport Biophysics, No.75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Lukáš Alán, Katarína Smolková, Eva Kronusová, Jitka Šantorová & Petr Ježek

  2. Dept. No.75, Membrane Transport Biophysics, Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 14220, Prague 4, Czech Republic

    Petr Ježek

Authors
  1. Lukáš Alán
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  2. Katarína Smolková
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  3. Eva Kronusová
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  4. Jitka Šantorová
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  5. Petr Ježek
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Corresponding author

Correspondence to Petr Ježek.

Additional information

Lukáš Alán and Katarína Smolková contributed equally to this work.

Eva Kronusová deceased in 2006

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Alán, L., Smolková, K., Kronusová, E. et al. Absolute levels of transcripts for mitochondrial uncoupling proteins UCP2, UCP3, UCP4, and UCP5 show different patterns in rat and mice tissues. J Bioenerg Biomembr 41, 71–78 (2009). https://doi.org/10.1007/s10863-009-9201-2

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  • DOI: https://doi.org/10.1007/s10863-009-9201-2

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