Recently, a series of structure determinations has nearly completed a structural description of the transport cycle of the sarcoplasmic reticulum Ca2+-ATPase, especially those steps concerned with the phosphorylation by ATP and the dephosphorylation reaction. From these structures Ca2+-ATPase emerges as a molecular machine, where globular cytosolic domains and transmembrane helices work in concert like a mechanical pump, as can be vividly demonstrated in animated versions of the pump cycle. The structures show that both ATP phosphorylation and dephosphorylation at Asp351 take place as nucleophilic SN2 reactions, which are associated with Ca2+ and H+ occluded states, respectively. These transitory steps ensure efficient coupling between Ca2+ transport and ATP hydrolysis.
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Andersen, J. P., Lassen, K., and Mòller, J. V. (1985). J. Biol. Chem. 260, 371–380.
Axelsen, K. B., and Palmgren, M. G. (1998). J. Mol. Evol. 46, 84–101.
Clausen, J. D., Vilsen, B., McIntosh, D. B., Einholm, A. P., and Andersen, J. P. (2004). Proc. Natl. Acad. Sci. 101, 2776–2781.
Coan, C., Ji, J.-Y., and Amaral, J. A. (1994). Biochemistry 33, 3722–3731.
Cornelius, F., and Mòller, J. V. (1991). FEBS Lett. 284, 46–50.
Danko, S., Kamidochi, M., Daiho, T., Yamasaki, K., Suzuki, H., and Toyoshima, C. (2001). FEBS Lett. 505, 129–135.
Danko, S., Yamasaki, K., Daiho, T., and Suzuki, H. (2004). J. Biol. Chem. 279, 14991–14998.
Dupont, Y. (1980). Eur. J. Biochem. 109, 231–238.
Forge, V., Mintz, E., and Guillain, F. (1993). J. Biol. Chem. 268, 10953–10968.
Hua, S., Inesi, G., Nomura, H., and Toyoshima, C. (2002). Biochemistry 41, 11405–11410.
Lenoir, G., Picard, M., Gauron, C., Montigny, C., le Marechal, P., Falson, P., le Maire, M., Mòller, J. V., and Champeil, P. (2004). J. Biol. Chem. 279, 9156–9166.
Levy, D., Seigneuret, M., Bluzat, A., and Rigaud, J.-L. (1990). J. Biol. Chem. 265, 19524–19534.
Lutsenko, S., and Kaplan, J. H. (1995). Biochemistry 34, 15607–15613.
Mòller, J. V., Juul, B., and le Maire, M. (1996). Biochim. Biophys. Acta 1286, 1–51.
Mòller, J. V., Lenoir, G., Marchand, C., Montigny, C., le Maire, M., Toyoshima, C., Juul, B. S., and Champeil, P. (2002). J. Biol. Chem. 277, 38647–38659.
Montigny, C., Jaxel, C., Shainskaya, A., Vinh, J., Labas, V., Mòller, J. V., Karlish, S. J. D., and le Maire, M. (2004). J. Biol. Chem. 279, 43971–43981.
Olesen, C., Sòrensen, T. L.-M., Nielsen, R. C., Mòller, J. V., and Nissen, P. (2004). Science 306, 2251–2255.
Orlowski, S., and Champeil, P. (1991). Biochemistry 30, 352– 361.
Picard, M., Toyoshima, C., and Champeil, P. (2005). J. Biol. Chem. 280, 18745–18754.
Shigekawa, M., and Pearl, M. (1976). J. Biol. Chem. 251, 6947– 6952.
Sòrensen, T. L.-M., Mòller, J. V., and Nissen, P. (2004a). Science 304, 1672–1675.
Sòrensen, T. L.-M., Clausen, J. D., Jensen, A.-M. L., Vilsen, B., Mòller, J. V., Andersen, J. P., and Nissen, P. (2004b). J. Biol. Chem. 279, 46355–46358.
Sugita, Y., Miyashita, N., Ikeguchi, M., Kidera, A., and Toyoshima, C. (2005). J. Am. Chem. Soc. 127, 6150–6151.
Takisawa, H., and Makinose, M. (1983). J. Biol. Chem. 258, 2986– 2992.
Tanford, C. (1983). Annu. Rev. Biochem. 52, 379–409.
Toyoshima, C., and Mizutani, T. (2004). Nature 430, 529–535.
Toyoshima, C., Nakasako, M., Nomura, H., and Ogawa, H. (2000). Nature 405, 647–655.
Toyoshima, C., and Nomura, H. (2002). Nature 418, 605–611.
Toyoshima, C., Nomura, H., and Tsuda, T. (2004). Nature 432, 361–368.
Troullier, A., Girardet, J.-L., and Dupont, Y. (1992). J. Biol. Chem. 267, 22821–22829.
Vilsen, B., and Andersen, J. P. (1992). J. Biol. Chem. 267, 3539–3550.
Wakabayashi, S., and Shigekawa, M. (1990). Biochemistry 29, 7309–7318.
Wang, G., Yamasaki, K., Daiho, T., and Suzuki, H. (2005). J. Biol. Chem. 280, 26508–26576.
White, S. (2005). Membrane proteins of known 3D structure. Available at http://blanco.biomol.uci.edu.
Yü, X., Carroll, S., Rigaud, J.-L., and Inesi, G. (1993). Biophys. J. 64, 1232–1242.
Yu, X., Hao, L., and Inesi, G. (1994). J. Biol. Chem. 269, 16656–16661.
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Møller, J.V., Olesen, C., Jensen, AM.L. et al. The Structural Basis for Coupling of Ca2+ Transport to ATP Hydrolysis by the Sarcoplasmic Reticulum Ca2+-ATPase. J Bioenerg Biomembr 37, 359–364 (2005). https://doi.org/10.1007/s10863-005-9471-2
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DOI: https://doi.org/10.1007/s10863-005-9471-2