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13Cα CEST experiment on uniformly 13C-labeled proteins

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An Erratum to this article was published on 11 February 2015

Abstract

A new HSQC-based 13Cα CEST pulse scheme is proposed, which is suitable for uniformly 13C- or 13C, 15N-labeled samples in either water or heavy water. Except for Thr and Ser residues, the sensitivity of this scheme for uniformly labeled samples is similar to that of the previous scheme for selectively 13Cα-labeled samples with 100 % isotope enrichment. The experiment is demonstrated on an acyl carrier protein domain. Our 13Cα CEST data reveal that the minor state of the acyl carrier protein has high helical propensity. The new scheme will facilitate structural characterization of invisible minor states.

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Acknowledgments

This work is supported by a Grant from Singapore Ministry of Education Academic Research Fund Tier 2 (MOE2012-T2-2-138).

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Authors and Affiliations

  1. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117543, Singapore

    Yang Zhou & Daiwen Yang

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  1. Yang Zhou
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  2. Daiwen Yang
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Correspondence to Daiwen Yang.

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Zhou, Y., Yang, D. 13Cα CEST experiment on uniformly 13C-labeled proteins. J Biomol NMR 61, 89–94 (2015). https://doi.org/10.1007/s10858-014-9888-1

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