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On the use of ultracentrifugal devices for sedimented solute NMR

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Abstract

We have recently proposed sedimented solute NMR (SedNMR) as a solid-state method to access biomolecules without the need of crystallization or other sample manipulation. The drawback of SedNMR is that samples are intrinsically diluted and this is detrimental for the signal intensity. Ultracentrifugal devices can be used to increase the amount of sample inside the rotor, overcoming the intrinsic sensitivity limitation of the method. We designed two different devices and we here report the directions for using such devices and the relevant equations for determining the parameters for sedimentation.

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Acknowledgments

This work has been supported by the EC contracts East-NMR no. 228461 and Bio-NMR no. 261863, INSTRUCT (European FP7 e-Infrastructure grant, contract no. 211252, http://www.instruct-fp7.eu/), and Ente Cassa Risparmio Firenze. Fruitful discussion with Anja Böckmann (IBCP, Lyon) is acknowledged. We are grateful to Alberto Catelani, Massimo Falorsi, Massimo Merciai and Nicola Pasqualetti (Department of Physics and Astronomy, University of Florence, Italy) for the production of device 2.

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Authors and Affiliations

  1. Center for Magnetic Resonance (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy

    Ivano Bertini, Leonardo Gonnelli, Claudio Luchinat & Enrico Ravera

  2. Fondazione Farmacogenomica FiorGen onlus, Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy

    Ivano Bertini & Claudio Luchinat

  3. Giotto Biotech, Via Madonna del Piano 6, 50019, Sesto Fiorentino, FI, Italy

    Ivano Bertini

  4. Bruker Biospin GmbH, Silberstreifen 4, 76287, Rheinstetten, Germany

    Frank Engelke, Benno Knott & David Osen

  5. Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, FI, Italy

    Claudio Luchinat & Enrico Ravera

Authors
  1. Ivano Bertini
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  2. Frank Engelke
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  3. Leonardo Gonnelli
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  4. Benno Knott
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  5. Claudio Luchinat
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  6. David Osen
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  7. Enrico Ravera
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Corresponding author

Correspondence to Claudio Luchinat.

Additional information

Prof. Dr. Ivano Bertini passed away on July 7th, 2012.

CAD drawings for the production of device 2 are available upon request. If appropriate the authors provide service for filling rotors.

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Bertini, I., Engelke, F., Gonnelli, L. et al. On the use of ultracentrifugal devices for sedimented solute NMR. J Biomol NMR 54, 123–127 (2012). https://doi.org/10.1007/s10858-012-9657-y

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  • DOI: https://doi.org/10.1007/s10858-012-9657-y

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