Skip to main content
Log in

The insect defensin lucifensin from Lucilia sericata

  • NMR structure note
  • Published:
Journal of Biomolecular NMR Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

References

  • Andersen AS, Sandvang D, Schnorr KM, Kruse T, Neve S, Joergensen B, Karlsmark T, Krogfelt KA (2010) A novel approach to the antimicrobial activity of maggot debridement therapy. J Antimicrob Chemother 65(8):1646–1654

    Article  Google Scholar 

  • Bulet P, Cociancich S, Reuland M, Sauber S, Bischoff R, Hegy G, Van Dors-selaer A, Hetru C, Hoffmann JA (1992) A novel insect defensin mediates the inducible antibacterial activity in larvae of the dragon fly Aeschna cyanea (Paleoptera, odonata). Eur J Biochem 209(3):977–984

    Article  Google Scholar 

  • Čeřovský V, Ždárek J, Fučík V, Monincová L, Voburka V, Bém R (2009) Lucifensin, the long-sought antimicrobial factor of medicinal maggots of the blowfly Lucilia sericata. Cell Mol Life Sci 67(3):455–466

    Google Scholar 

  • Cociancich S, Ghazi A, Hetru C, Hoffmann JA, Letellier L (1993) Insect defensin, an inducible antibacterial peptide, forms voltage-dependent channels in Micrococcus luteus. J Biol Chem 268(26):19239–19245

    Google Scholar 

  • Cornet B, Bonmatin J, Hetru C, Hoffmann JA, Ptak M, Vovelle F (1995) Refined three-dimensional solution structure of insect defensin A. Structure 3(5):435–448. PDB entry: 1ICA

    Google Scholar 

  • Dumville JC, Worthy G, Bland JM, Cullum N, Dowson C, Iglesias C, Mitchell JL, Nelson EA, Soares MO, Torgerson DJ, VenUS II Team (2009) Larval therapy for leg ulcers (VenUS II): randomised controlled trial. BMJ 338:b773

  • Essmann U, Perera L, Berkowitz ML, Darden T, Lee H, Pedersen LG (1995) A smooth particle mesh Ewald method. J Chem Phys 103(19):8577

    Article  ADS  Google Scholar 

  • Hanzawa H, Shimada I, Kuzuhara T, Komano H, Kohda D, Inagaki F, Natori S, Arata Y (1990) 1H nuclear magnetic resonance study of the solution conformation of an antibacterial protein, sapecin. FEBS Lett 269:413-420. PDB entry: 1L4 V

    Google Scholar 

  • Jaklic D, Lapanje A, Zupancic K, Smrke D, Gunde-Cimerman N (2008) Selective antimicrobial activity of maggots against pathogenic bacteria. J Med Microbiol 57(Pt 5):617–625

    Article  Google Scholar 

  • Keller R (2004) The computer aided resonance assignment tutorial, 1st edn. CANTINA Verlag, Goldau (Switzerland)

  • Kerridge A, Lappin-Scott H, Stevens JR (2005) Antibacterial properties of larval secretions of the blowfly, Lucilia sericata. Med Vet Entomol 19:333–337

    Article  Google Scholar 

  • Konagurthu AS, Whisstock JC, Stuckey PJ, Lesk AM (2006) MUSTANG: a multiple structural alignment algorithm. Proteins 64:559–574

    Article  Google Scholar 

  • Krieger E, Koraimann G, Vriend G (2002) Increasing the precision of comparative models with YASARA NOVA—a self-parameterizing forcefield. Proteins 47:393–402

    Article  Google Scholar 

  • Krieger E, Joo K, Lee J, Lee J, Raman S, Thompson J, Tyka M, Baker D, Karplus K (2009) Improving physical realism, stereochemistry and side-chain accuracy in homology modeling: four approaches that performed well in CASP8. Proteins 77(9):114–122

    Article  Google Scholar 

  • Laskowski R, Rullmann J, MacArthur M, Kaptein R, Thornton J (1996) AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J Biomol NMR 8(4):477–486

    Article  Google Scholar 

  • López-Méndez B, Güntert P (2006) Automated protein structure determination from NMR spectra. J Am Chem Soc 128:13112–13122

    Article  Google Scholar 

  • Mygind PH, Fischer RL, Schnorr KM, Hansen MT, Sönksen CP, Ludvigsen S, Raventós D, Buskov S, Christensen B, De Maria L, Taboureau O, Yaver D, Elvig-Jørgensen SG, Sørensen MV, Christensen BE, Kjaerulff S, Frimodt-Moller N, Lehrer RI, Zasloff M, Kristensen HH (2005) Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus. Nature 437(7061):975–980

    Article  ADS  Google Scholar 

  • Schneider T, Kruse T, Wimmer R, Wiedemann I, Sass V, Pag U, Jansen A, Nielsen AK, Mygind PH, Raventos DS, Neve S, Ravn B, Bon-vin AMJJ, De Maria L, Andersen AS, Gammelgaard LK, Sahl H, Kristensen H (2010) Plectasin, a fungal defensin, targets the bacterial cell wall precursor lipid II. Science 328:1168–1172

    Article  ADS  Google Scholar 

  • Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44(4):213–223

    Article  Google Scholar 

  • Takeuchi K, Takahashi H, Sugai M, Iwai H, Kohno T, Sekimizu K, Natori S, Shimada I (2004) Channel-forming membrane permeabilization by an antibacterial protein, sapecin. J Biol Chem 279:4981–4987

    Article  Google Scholar 

Download references

Acknowledgments

The project is part of the DanCARD center funded by the Danish Council for Strategic Research. We would like to acknowledge the Carlsberg Research Center for use of the 800 MHz spectrometer and in that respect thank Bent Pedersen and Sebastian Meier for technical assistance with recording of the NMR spectra. We would also like to thank Charlotte Steen for aiding with MALDI-TOF MS. The NMR laboratory at Aalborg University is supported by the Obel and SparNord foundations. Discovery and recombinant production of Lucifensin was supported by a grant from the Danish Research Council for Technology and Production (grant number 274-05-0435 to K. A. K).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Reinhard Wimmer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nygaard, M.K.E., Andersen, A.S., Kristensen, HH. et al. The insect defensin lucifensin from Lucilia sericata . J Biomol NMR 52, 277–282 (2012). https://doi.org/10.1007/s10858-012-9608-7

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10858-012-9608-7

Keywords

Navigation