Abstract
We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.
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Acknowledgements
The facilities provided by National Facility for High Field NMR at TIFR supported by Department of Science and Technology (DST), Department of Biotechnology (DBT), Council of Scientific and Industrial Research (CSIR), and Tata Institute of Fundamental Research, Mumbai, India, and NMR Research Centre at IISc supported by DST are gratefully acknowledged. HSA acknowledges support from Department of Atomic Energy (DAE) BRNS and DST-FAST TRACK research awards. We thank Prof. Alok Bhattacharya (Jawaharlal Nehru University, New Delhi), for providing us the Eh-CaBP expression plasmid. We thank Mr. Dinesh Kumar for urea-denatured dsmt3 sample.
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Barnwal, R.P., Rout, A.K., Atreya, H.S. et al. Identification of C-terminal neighbours of amino acid residues without an aliphatic 13Cγ as an aid to NMR assignments in proteins. J Biomol NMR 41, 191–197 (2008). https://doi.org/10.1007/s10858-008-9254-2
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DOI: https://doi.org/10.1007/s10858-008-9254-2