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Cross-correlated spin relaxation effects in methyl 1H CPMG-based relaxation dispersion experiments: Complications and a simple solution

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Abstract

Artifacts associated with the measurement of methyl 1H single quantum CPMG-based relaxation dispersion profiles are described. These artifacts arise due to the combination of cross-correlated spin relaxation effects involving intra-methyl 1H–1H dipolar interactions and imperfections in 1H refocusing pulses that are applied during CPMG intervals that quantify the effects of chemical exchange on measured transverse relaxation rates. As a result substantial errors in extracted exchange parameters can be obtained. A simple ‘work-around’ is presented where the 1H chemical shift difference between the exchanging states is extracted from a combination of 13C single quantum and 13C–1H multiple quantum dispersion profiles. The approach is demonstrated with an application to a folding/unfolding reaction involving a G48M mutant Fyn SH3 domain.

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Authors and Affiliations

  1. Contribution from the Protein Engineering Network Centers of Excellence and the Departments of Medical Genetics, Biochemistry and Chemistry, The University of Toronto, Toronto, Ontario, Canada,, M5S 1A8

    Dmitry M. Korzhnev, Anthony K. Mittermaier & Lewis E. Kay

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  1. Dmitry M. Korzhnev
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  2. Anthony K. Mittermaier
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  3. Lewis E. Kay
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Correspondence to Lewis E. Kay.

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Korzhnev, D.M., Mittermaier, A.K. & Kay, L.E. Cross-correlated spin relaxation effects in methyl 1H CPMG-based relaxation dispersion experiments: Complications and a simple solution. J Biomol NMR 31, 337–342 (2005). https://doi.org/10.1007/s10858-005-2468-7

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  • DOI: https://doi.org/10.1007/s10858-005-2468-7

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