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Spin-state selection for increased confidence in cross-correlation rates measurements

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Abstract

A new approach is described for measuring chemical shift anisotropy (CSA)/dipolar cross-correlated relaxation (CCR) rates based on the selection of the individual 15N doublet components prior to the relaxation period. The method uses the spin-state-selective element (S3E) of Sørensen and co-authors [Meissner et al. (1997) J. Mag. Reson., 128, 92–97]. The main advantage of the new method compared to other J-resolved experiments is that it does not create problems of additional signal overlap encountered in coupled spectra. At the same time, this approach allows a simpler control of magnetization pathways than the indirect methods. The method is demonstrated for the B3 domain of protein G.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, 1115 Agriculture/Life Sciences, Surge Bldg.(#296), College Park, 20742-3360, MD, U.S.A.

    Paul R. Vasos, Jennifer B. Hall & David Fushman

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  1. Paul R. Vasos
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  2. Jennifer B. Hall
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  3. David Fushman
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Correspondence to David Fushman.

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Vasos, P.R., Hall, J.B. & Fushman, D. Spin-state selection for increased confidence in cross-correlation rates measurements. J Biomol NMR 31, 149–154 (2005). https://doi.org/10.1007/s10858-004-7562-8

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  • DOI: https://doi.org/10.1007/s10858-004-7562-8

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