Skip to main content

Advertisement

SpringerLink
Log in

PLGA film/Titanium nanotubues as a sustained growth factor releasing system for dental implants

  • Delivery Systems
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Ti-based implants sometimes fail to integrate with surrounding bone tissue due to insufficiency of new bone formation and surface bonding. To overcome this problem, this research focused on establishing a sustained bone growth factor delivery system by applying anodized TiO2 nanotube arrays and PLGA film on the titanium implant surface. TiO2 nanotube arrays were made by anodic oxidation method, and were then filled with rhBMP2 by vacuum freeze-drying. Next, PLGA was deposition on the surface of this material. The designed system was characterized, pharmacokinetic release rate of rhBMP2 was determined. Adhesion, proliferation, and differentiation activity of osteoblasts cultured on the new surfaces and traditional titanium surfaced were compared. SEM showed that a surface of TiO2 nanotube arrays were successfully generated. PLGA membranes of 50 nm, 250 nm, 800 nm thickness were successfully deposited on the surfaces of TiO2 nanotube layers by using 1%, 3%, 10% PLGA solutions. PLGA film of 250 nm thickness showed ideally controlled release of rhBMP2, lasting for 4 weeks. Furthermore, 250 nm thickness PLGA film improved osteoblast adhesion, proliferation, and levels of alkaline phosphatase. In conclusion, the PLGA film / TiO2 nanotube growth factor delivery system can effectively sustain the release of rhBMP-2, and promote proliferation and differentiation of MC3T3-E1 osteoblasts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Niinomi M. Mechanical biocompatibilities of titanium alloys for biomedical applications. J Mech Behav Biomed Mater. 2008;1(1):30–42.

    Article  Google Scholar 

  2. Ardura JA, Portal‐Núñez S, Lozano D, Gutiérrez‐Rojas I, Sánchez‐Salcedo S, López‐Herradón A, et al. Local delivery of parathyroid hormone‐related protein‐derived peptides coated onto a hydroxyapatite‐based implant enhances bone regeneration in old and diabetic rats. J Biomed Mater Res A. 2016;104(8):2060–70.

    Article  CAS  Google Scholar 

  3. Oates TW, Galloway P, Alexander P, Green AV, Huynh-Ba G, Feine J, et al. The effects of elevated hemoglobin A 1c in patients with type 2 diabetes mellitus on dental implants. J Am Dent Assoc. 2014;145(12):1218–26.

    Article  CAS  Google Scholar 

  4. Gómez‐Moreno G, Aguilar‐Salvatierra A, Rubio Roldán J, Guardia J, Gargallo J, Calvo‐Guirado JL. Peri‐implant evaluation in type 2 diabetes mellitus patients: a 3‐year study. Clin Oral Implants Res. 2015;26(9):1031–5.

    Article  Google Scholar 

  5. Bagherifard S, Ghelichi R, Khademhosseini A, Guagliano M. Cell response to nanocrystallized metallic substrates obtained through severe plastic deformation. ACS Appl Mater & Interfaces. 2014;6(11):7963–85.

    Article  CAS  Google Scholar 

  6. Kalemaj Z, Scarano A, Valbonetti L, Rapone B, Grassi FR. Bone Response to Four Dental Implants with Different Surface Topographies: A Histologic and Histometric Study in Minipigs. Int J Periodontics & Restor Dent. 2016;36(5):745–54.

    Article  Google Scholar 

  7. Liu W, Su P, Chen S, Wang N, Wang J, Liu Y, et al. Antibacterial and osteogenic stem cell differentiation properties of photoinduced TiO2 nanoparticle-decorated TiO2 nanotubes. Nanomedicine. 2015;10(5):713–23.

    Article  CAS  Google Scholar 

  8. Balasundaram G, Yao C, Webster TJ. TiO2 nanotubes functionalized with regions of bone morphogenetic protein‐2 increases osteoblast adhesion. J Biomed Mater Res A. 2008;84(2):447–53.

    Article  Google Scholar 

  9. Lee K, Mazare A, Schmuki P. One-dimensional titanium dioxide nanomaterials: nanotubes. Chemical Reviews. 2014;114(19):9385–454.

    Article  CAS  Google Scholar 

  10. Salou L, Hoornaert A, Louarn G, Layrolle P. Enhanced osseointegration of titanium implants with nanostructured surfaces: an experimental study in rabbits. Acta Biomater. 2015;11:494–502.

    Article  CAS  Google Scholar 

  11. Justin D, Jin S, Frandsen C, Brammer K, Bjursten L, Oh S, et al. In vitro and in vivo evaluation of implant surfaces treated with titanium oxide (TiO2) nanotube arrays to enhance osseointegration between arthroplasty implants and surrounding bone. Bone Jt J. 2016;98(SUPP8):71.

    Google Scholar 

  12. Brammer KS, Frandsen CJ, Jin S. TiO 2 nanotubes for bone regeneration. Trends Biotechnol. 2012;30(6):315–22.

    Article  CAS  Google Scholar 

  13. Kumeria T, Mon H, Aw MS, Gulati K, Santos A, Griesser HJ, et al. Advanced biopolymer-coated drug-releasing titania nanotubes (TNTs) implants with simultaneously enhanced osteoblast adhesion and antibacterial properties. Colloids Surf B: Biointerfaces. 2015;130:255–63.

    Article  CAS  Google Scholar 

  14. Lee J-K, Cho L-R, Um H-S, Chang B-S, Cho K-S. Bone formation and remodeling of three different dental implant surfaces with Escherichia coli-derived recombinant human bone morphogenetic protein 2 in a rabbit model. Int J Oral & Maxillofac Implants. 2013;28(2):424–30.

    Article  Google Scholar 

  15. James AW, LaChaud G, Shen J, Asatrian G, Nguyen V, Zhang X, et al. A review of the clinical side effects of bone morphogenetic protein-2. Tissue Eng Part B: Rev. 2016;22(4):284–97.

    Article  CAS  Google Scholar 

  16. Zara JN, Siu RK, Zhang X, Shen J, Ngo R, Lee M, et al. High doses of bone morphogenetic protein 2 induce structurally abnormal bone and inflammation in vivo. Tissue Eng Part A. 2011;17(9-10):1389–99.

    Article  CAS  Google Scholar 

  17. Yang HS, La W-G, Bhang SH, Jeon J-Y, Lee JH, Kim B-S. Heparin-conjugated fibrin as an injectable system for sustained delivery of bone morphogenetic protein-2. Tissue Eng Part A. 2010;16(4):1225–33.

    Article  CAS  Google Scholar 

  18. Bai Y, Yin G, Huang Z, Liao X, Chen X, Yao Y, et al. Localized delivery of growth factors for angiogenesis and bone formation in tissue engineering. Int Immunopharmacol. 2013;16(2):214–23.

    Article  CAS  Google Scholar 

  19. Bae IH, Yun KD, Kim HS, Jeong BC, Lim HP, Park SW, et al. Anodic oxidized nanotubular titanium implants enhance bone morphogenetic protein‐2 delivery. J Biomed Mater Res Part B Appl Biomater. 2010;93(2):484–91.

    Article  Google Scholar 

  20. Xu Y, Kim CS, Saylor DM, Koo D. Polymer degradation and drug delivery in PLGA‐based drug–polymer applications: A review of experiments and theories. J Biomed Mater Res Part B Appl Biomater. 2016;105(6):1692–716.

    Article  Google Scholar 

  21. Qi R, Guo R, Zheng F, Liu H, Yu J, Shi X. Controlled release and antibacterial activity of antibiotic-loaded electrospun halloysite/poly (lactic-co-glycolic acid) composite nanofibers. Colloids Surf B: Biointerfaces. 2013;110:148–55.

    Article  CAS  Google Scholar 

  22. Meng ZX, Xu XX, Zheng W, Zhou HM, Li L, Zheng YF, et al. Preparation and characterization of electrospun PLGA/gelatin nanofibers as a potential drug delivery system. Colloids Surf B: Biointerfaces. 2011;84(1):97–102.

    Article  CAS  Google Scholar 

  23. Zodrow KR, Schiffman JD, Elimelech M. Biodegradable polymer (PLGA) coatings featuring cinnamaldehyde and carvacrol mitigate biofilm formation. Langmuir. 2012;28(39):13993–9.

    Article  CAS  Google Scholar 

  24. Meng H-W, Chien EY, Chien H-H. Dental implant bioactive surface modifications and their effects on osseointegration: a review. Biomark Res. 2016;4(1):24.

    Article  Google Scholar 

  25. W-q Yu, Qiu J, Zhang F-q. In vitro corrosion study of different TiO 2 nanotube layers on titanium in solution with serum proteins. Colloids Surf B: Biointerfaces. 2011;84(2):400–5.

    Article  Google Scholar 

  26. Samadi N, Abbadessa A, Di Stefano A, Van Nostrum C, Vermonden T, Rahimian S, et al. The effect of lauryl capping group on protein release and degradation of poly (d, l-lactic-co-glycolic acid) particles. J Control Release. 2013;172(2):436–43.

    Article  CAS  Google Scholar 

  27. Kim BS, Kang H-J, Park J-Y, Lee J. Fucoidan promotes osteoblast differentiation via JNK-and ERK-dependent BMP2–Smad 1/5/8 signaling in human mesenchymal stem cells. Exp Mol Med. 2015;47(1):e128.

    Article  CAS  Google Scholar 

Download references

Funding

This study was funded by Shanghai Leading Academic Discipline Project (S30206 and T0202), Science and Technology committee of Shanghai (08DZ2271100, ZZjdyx13107, and 15XD1502500), National Natural Science Funds of China (81300912), Shandong Provincial Natural Science Foundation, China (ZR2017BH030), and China Postdoctoral Science Foundation (2014M561942).

Author information

Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Oral Biomedicine, College of Stomatology, Shandong University, No. 44-1, Wenhuaxilu Rd, Jinan, Shandong, 250012, People’s Republic of China

    Shengjun Sun

  2. Department of Prosthodontics, Ninth People’s Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, No. 639, zhizaoju Rd, Shanghai, 250011, People’s Republic of China

    Shengjun Sun, Yilin Zhang, Deliang Zeng, Songmei Zhang, Fuqiang Zhang & Weiqiang Yu

  3. Department of Stomatology, Shandong Provincial Hospital affiliated to Shandong University, Yanshanxi Rd, Jinan, Shandong, 250001, People’s Republic of China

    Yilin Zhang

  4. Eastman Institute for Oral Health, University of Rochester, 625 Elmwood Avenue, Rochester, New York, 14620, USA

    Songmei Zhang

Authors
  1. Shengjun Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yilin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Deliang Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Songmei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fuqiang Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Weiqiang Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Fuqiang Zhang or Weiqiang Yu.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, S., Zhang, Y., Zeng, D. et al. PLGA film/Titanium nanotubues as a sustained growth factor releasing system for dental implants. J Mater Sci: Mater Med 29, 141 (2018). https://doi.org/10.1007/s10856-018-6138-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10856-018-6138-1

Search

Navigation