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Effect of synthetic and natural-derived novel endodontic irrigant solutions on mechanical properties of human dentin

  • Clinical Applications of Biomaterials
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

This study aimed to evaluate the effects of different synthetic and natural-derived root canal irrigants (6% sodium hypochlorite [NaOCl], 6% calcium hypochlorite [Ca(OCl)2] and 6.5% grape seed extract [GSE]) on dentin mechanical properties (flexural strength, ultimate tensile strength [UTS] and fracture resistance). Rectangular-shaped beams and hourglass-shaped sections obtained from mid-coronal and root dentin were treated with 6% NaOCl, 6% Ca(OCl)2 or 6.5% GSE for 30 min. The irrigant solutions were replaced every 5 min. Then, the dentin specimens were rinsed with distilled water (DW) followed by incubation with 17% EDTA for 1 min, and thoroughly rinsed with DW again. Specimens from the control group were tested without prior irrigation. After treatment with the irrigants, dentin beams were used to assess the flexural strength (n = 10) while UTS was evaluated using the root dentin hourglass-shaped sections (n = 10). Similarly, roots with 1 mm of dentinal wall thickness were obtained from human teeth and treated with the same irrigant solutions (n = 10). A compressive loading was applied to the coronal surfaces of roots until fracture. The values of each mechanical test were statistically analyzed individually by one-way ANOVA followed by Tukey HSD test (P < 0.05). NaOCl significantly reduced the mechanical properties of dentin in all mechanical tests (P < 0.05) and no statistical difference was found among Ca(OCl)2, GSE and control group (P > 0.05). It can be concluded that Ca(OCl)2 and GSE may be alternative irrigant solutions, since they do not negatively affect the dentin mechanical properties.

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Acknowledgements

We have no financial affiliation (e.g., employment, direct payment, stock holdings, retainers, consultancies, patent licensing arrangements, or honoraria) or involvement with any commercial organization with direct financial interest in the subject or materials discussed in this manuscript, neither have any such arrangements existed in the past 3 years.

Funding

This investigation was supported by research grants from Cnpq (Grant # 122244/2015-0) and FAPERGS (Grant # 0413-2551/15-5).

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Authors and Affiliations

  1. Department of Restorative Dentistry, Dental School of Universidade de Passo Fundo, Passo Fundo, RS, Brazil

    Doglas Cecchin, Vitória Soares Giaretta, Bérove Granella Cadorin, Matheus Albino Souza & Ana Paula Farina

  2. Department of Operative Dentistry, College of Dentistry and Dental Clinics, University of Iowa, Iowa City, IA, USA

    Cristina de Mattos Pimenta Vidal

Authors
  1. Doglas Cecchin
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  2. Vitória Soares Giaretta
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  3. Bérove Granella Cadorin
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  4. Matheus Albino Souza
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  5. Cristina de Mattos Pimenta Vidal
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  6. Ana Paula Farina
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Corresponding author

Correspondence to Doglas Cecchin.

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Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants or animals performed by any of the authors. Extracted human teeth were used in this study based on a protocol approved by the University of Passo Fundo ethics committee (protocols: No. 086047/2015 and No. 086051/2015).

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Informed consent was not obtained because the study was considered non-human subjects research.

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Cecchin, D., Soares Giaretta, V., Granella Cadorin, B. et al. Effect of synthetic and natural-derived novel endodontic irrigant solutions on mechanical properties of human dentin. J Mater Sci: Mater Med 28, 141 (2017). https://doi.org/10.1007/s10856-017-5960-1

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  • DOI: https://doi.org/10.1007/s10856-017-5960-1

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