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Effect of bioactive dental adhesive on periodontal and endodontic pathogens

  • Biomaterials Synthesis and Characterization
  • Original Research
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Abstract

The objectives of this study were to: (1) develop a new bioactive dental bonding agent with nanoparticles of amorphous calcium phosphate and dimethylaminohexadecyl methacrylate for tooth root caries restorations and endodontic applications, and (2) investigate biofilm inhibition by the bioactive bonding agent against eight species of periodontal and endodontic pathogens for the first time. Bonding agent was formulated with 5 % of dimethylaminohexadecyl methacrylate. Nanoparticles of amorphous calcium phosphate at 30 wt% was mixed into adhesive. Eight species of biofilms were grown on resins: Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Parvimonas micra, Enterococcus faecalis, Enterococcus faecium. Colony-forming units, live/dead assay, biomass, metabolic activity and polysaccharide of biofilms were determined. The results showed that adding dimethylaminohexadecyl methacrylate and nanoparticles of amorphous calcium phosphate into bonding agent did not decrease dentin bond strength (P > 0.1). Adding dimethylaminohexadecyl methacrylate reduced the colony-forming units of all eight species of biofilms by nearly three orders of magnitude. The killing efficacy of dimethylaminohexadecyl methacrylate resin was: P. gingivalis > A. actinomycetemcomitans > P. intermedia > P. nigrescens > F. nucleatum > P. micra > E. faecalis > E. faecium. Dimethylaminohexadecyl methacrylate resin had much less biomass, metabolic activity and polysaccharide of biofilms than those without dimethylaminohexadecyl methacrylate (P < 0.05). In conclusion, a novel dental adhesive was developed for root caries and endodontic applications, showing potent inhibition of biofilms of eight species of periodontal and endodontic pathogens, and reducing colony-forming units by three orders of magnitude. The bioactive adhesive is promising for tooth root restorations to provide subgingival margins with anti-periodontal pathogen capabilities, and for endodontic sealer applications to combat endodontic biofilms.

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Acknowledgments

This work was supported by NIH R01DE17974 (HX), National Science Foundation of China NSFC 81400487 (LW), 81200820 (XX), Youth Fund of Science and Technology Jilin Province 20150520043JH (LW), China Postdoctoral Foundation 2015M581405 (LW), China Scholarship Council (LW), NSFC 31328008 (LZ), NSF Guangdong 20130010014253 (LZ) and 2014A030313275 (LZ), Guangdong Science and Technology 2012B010200024 (LZ), and University of Maryland School of Dentistry bridging fund (HX).

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Authors and Affiliations

  1. VIP Integrated Department, Stomatological Hospital of Jilin University, Changchun, China

    Lin Wang

  2. Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, MD, 21201, USA

    Lin Wang, Xianju Xie, Michael D. Weir, Liang Zhao & Hockin H. K. Xu

  3. Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China

    Xianju Xie

  4. Department of Endodontics, University of North Carolina, Chapel Hill, NC, 27599, USA

    Ashraf F. Fouad

  5. Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, China

    Liang Zhao

  6. Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD, 21201, USA

    Hockin H. K. Xu

  7. Department of Mechanical Engineering, University of Maryland, Baltimore County, MD, 21250, USA

    Hockin H. K. Xu

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  1. Lin Wang
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  2. Xianju Xie
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Correspondence to Liang Zhao or Hockin H. K. Xu.

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Wang, L., Xie, X., Weir, M.D. et al. Effect of bioactive dental adhesive on periodontal and endodontic pathogens. J Mater Sci: Mater Med 27, 168 (2016). https://doi.org/10.1007/s10856-016-5778-2

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