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Evaluation of the osteogenesis and angiogenesis effects of erythropoietin and the efficacy of deproteinized bovine bone/recombinant human erythropoietin scaffold on bone defect repair

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

Erythropoietin (EPO) could promote the angiogenesis and may also play a role in bone regeneration. This study was conducted to evaluate the osteogenesis and angiogenesis effects of EPO and the efficacy of deproteinized bovine bone/recombinant human EPO scaffold on bone defect repair. Twenty-four healthy adult goats were chosen to build goat defects model and randomly divided into four groups. The goats were treated with DBB/rhEPO scaffolds (group A), porous DBB scaffolds (group B), autogenous cancellous bone graft (group C), and nothing (group D). Animals were evaluated with radiological and histological methods at 4, 8 and 12 weeks after surgery. The grey value of radiographs was used to evaluate the healing of the defects and the outcome revealed that the group A had a better outcome of defect healing compared with group B (P < 0.05). However, the grey values in group A were lower than group C at week 4 and week 8 (P < 0.05), but at week 12 their difference had no statistical significance (P > 0.05). The newly formed bone area was calculated from histological sections and the results demonstrated that the amount of new bone in group A increased significantly compared with that in group B (P < 0.05) but was inferior to that in group C (P > 0.05) at 4, 8, 12 weeks respectively. In addition, the expression of vascular endothelial growth factor (VEGF) by immunohistochemical testing and real-time polymerase chain reaction at 12 weeks in group A was significantly higher than that in group B (P < 0.05), and also better than that in group C at week 4 and week 8 (P < 0.05), but at week 12 their difference had no statistical significance (P > 0.05). Therefore, EPO has significant effects on bone formation and angiogenesis, and has capacity to promote the repair of bone defects. It is worthy of being recommended to further studies.

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Acknowledgments

This study was supported by the National Natural Science Fund of China (81271976/H0605 and 81171763).

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    Authors and Affiliations

    1. Department of Orthopaedics, West China Hospital, Sichuan University, 37# Wainan Guoxue Road, Chengdu, 610041, People’s Republic of China

      Donghai Li, Xiaowei Xie, Zhouyuan Yang & Pengde Kang

    2. Department of Orthopaedics, Hospital of Chengdu Office of People’s Government of Tibetan Autonomous Region, 20# Ximianqiaoheng Street, Chengdu, 610041, People’s Republic of China

      Liqing Deng

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    1. Donghai Li
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    Correspondence to Pengde Kang.

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    Donghai Li, Liqing Deng and Xiaowei Xie contributed equally to this work and should be regarded as first co-author.

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    Li, D., Deng, L., Xie, X. et al. Evaluation of the osteogenesis and angiogenesis effects of erythropoietin and the efficacy of deproteinized bovine bone/recombinant human erythropoietin scaffold on bone defect repair. J Mater Sci: Mater Med 27, 101 (2016). https://doi.org/10.1007/s10856-016-5714-5

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    • DOI: https://doi.org/10.1007/s10856-016-5714-5

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