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Substantivity of Ag–Ca–Si mesoporous nanoparticles on dentin and its ability to inhibit Enterococcus faecalis

  • Biocompatibility Studies
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Abstract

The main purpose of this study was to investigate the substantivity of Ag–Ca–Si mesoporous nanoparticles (Ag-MCSNs) on dentin and its residual antibacterial effects against Enterococcus faecalis. Ag-MCSNs were fabricated and characterized, ion release profile and pH were tested, and the ability to inhibit planktonic E. faecalis as well as the cytotoxicity was evaluated. Dentin slices were medicated with Ca(OH)2 paste, 2 % chlorhexidine gel and Ag-MCSNs paste for 7 days and then irrigated. Dentin slices were then immersed in E. faecalis suspension for 6 days and then transferred to fresh brain heart infusion solution. The optical density value within 10 h after immersing and transferring were measured and compared among groups. Results indicated that Ag-MCSNs showed high pH, sustained Ag+–Ca2+–SiO3 2− ion release, and high substantivity on dentin. The Ag-MCSNs exhibited strong antibacterial effects against planktonic E. faecalis and much better residual inhibition effects against E. faecalis growth on dentin than Ca(OH)2 paste (P < 0.05). The Ag-MCSNs showed excellent antibacterial ability against E. faecalis and high substantivity on dentin, which might be developed to a new effective intra-canal medicament for human teeth.

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References

  1. Nair PN. Pathogenesis of apical periodontitis and the causes of endodontic failures. Crit Rev Oral Biol Med. 2004;15:348–81.

    Article  Google Scholar 

  2. Carr GB, Schwartz RS, Schaudinn C, Gorur A, Costerton JW. Ultrastructural examination of failed molar retreatment with secondary apical periodontitis: an examination of endodontic biofilms in an endodontic retreatment failure. J Endod. 2009;35:1303–9.

    Article  Google Scholar 

  3. Love RM. Enterococcus faecalis–a mechanism for its role in endodontic failure. Int Endod J. 2001;34:399–405.

    Article  Google Scholar 

  4. Mohammadi Z, Shalavi S, Yazdizadeh M. Antimicrobial activity of calcium hydroxide in endodontics: a review. Chonnam Med J. 2012;48:133–40.

    Article  Google Scholar 

  5. Garcia-Caballero L, Quintas V, Prada-Lopez I, Seoane J, Donos N, Tomas I. Chlorhexidine substantivity on salivary flora and plaque-like biofilm: an in situ model. PLoS One. 2013;8:e83522.

    Article  Google Scholar 

  6. Rahimi S, Janani M, Lotfi M, Shahi S, Aghbali A, Vahid Pakdel M, et al. A review of antibacterial agents in endodontic treatment. Iran Endod J. 2014;9:161–8.

    Google Scholar 

  7. Mohammadi Z, Abbott PV. Antimicrobial substantivity of root canal irrigants and medicaments: a review. Aust Endod J. 2009;35:131–9.

    Article  Google Scholar 

  8. Kishen A. Advanced therapeutic options for endodontic biofilms. Endod Top. 2012;22:99–123.

    Article  Google Scholar 

  9. Moritz M, Geszke-Moritz M. Mesoporous materials as multifunctional tools in biosciences: principles and applications. Mater Sci Eng C. 2015;49C:114–51.

    Article  Google Scholar 

  10. Fan W, Wu C, Han P, Zhou Y, Xiao Y. Porous Ca-Si-based nanospheres: a potential intra-canal disinfectant-carrier for infected canal treatment. Mater Lett. 2012;81:16–9.

    Article  Google Scholar 

  11. Fan W, Wu D, Tay FR, Ma T, Wu Y, Fan B. Effects of adsorbed and templated nanosilver in mesoporous calcium-silicate nanoparticles on inhibition of bacteria colonization of dentin. Int J Nanomed. 2014;9:5217–30.

    Article  Google Scholar 

  12. Wu C, Chang J, Fan W. Bioactive mesoporous calcium-silicate nanoparticles with excellent mineralization ability, osteostimulation, drug-delivery and antibacterial properties for filling apex roots of teeth. J Mater Chem. 2012;22:16801–9.

    Article  Google Scholar 

  13. Fan W, Wu D, Ma T, Fan B. Ag-loaded mesoporous bioactive glasses against Enterococcus faecalis biofilm in root canal of human teeth. Dent Mater J. 2015;34:54–60.

    Article  Google Scholar 

  14. Kasraei S, Sami L, Hendi S, Alikhani MY, Rezaei-Soufi L, Khamverdi Z. Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus. Restor Dent Endod. 2014;39:109–14.

    Article  Google Scholar 

  15. Sre PRR, Reka M, Poovazhagi R, Kumar MA, Murugesan K. Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam. Spectrochim Acta A. 2014;135:1137–44.

    Google Scholar 

  16. Souza M, Cecchin D, Farina AP, Leite CE, Cruz FF, Pereira Cda C, et al. Evaluation of chlorhexidine substantivity on human dentin: a chemical analysis. J Endod. 2012;38:1249–52.

    Article  Google Scholar 

  17. Moazami F, Sahebi S, Jamshidi D, Alavi A. The long-term effect of calcium hydroxide, calcium-enriched mixture cement and mineral trioxide aggregate on dentin strength. Iran Endod J. 2014;9:185–9.

    Google Scholar 

  18. White JD, Lacefield WR, Chavers LS, Eleazer PD. The effect of three commonly used endodontic materials on the strength and hardness of root dentin. J Endod. 2002;28:828–30.

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant No. 81271130), Natural Science Foundation of Hubei Province, China (Grant No. 2013CFB241) and the Innovation Seed Fund of Wuhan University School of Medicine.

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    1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People’s Republic of China

      Wei Fan, Yujie Wu, Tengjiao Ma, Yanyun Li & Bing Fan

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    1. Wei Fan
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    2. Yujie Wu
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    Correspondence to Bing Fan.

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    Wei Fan and Yujie Wu are the co-first authors with equal contributions.

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    Fan, W., Wu, Y., Ma, T. et al. Substantivity of Ag–Ca–Si mesoporous nanoparticles on dentin and its ability to inhibit Enterococcus faecalis . J Mater Sci: Mater Med 27, 16 (2016). https://doi.org/10.1007/s10856-015-5633-x

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