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Novel implantable composite biomaterial by fibrin glue and amniotic membrane for ocular surface reconstruction

  • Biocompatibility Studies
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Abstract

Amniotic membrane transplantation (AMT) is considered a substantial treatment option in the management of ocular surface disorders. However, several inherent drawbacks still remain. The present study devised a novel implantable composite biomaterial of fibrin glue-double layer Amniotic membrane (AM) and evaluated the biomechanical properties and effects on corneal surface reconstruction in alkali-burned rabbit model. Biomechanic parameters were calculated by an electronic universal testing machine. Corneal alkali burning was done in the right eyes of thirty rabbits, which were randomized into three groups of ten animals each. The eyes in group 1 underwent fibrin glue-double layer AMT, the eyes in group 2 underwent ordinary single layer AMT, and the eyes in group 3 (control group) did not undergo any surgical procedure. Healing of corneal epithelial defect, extent of corneal vascularization and corneal clarity were assessed and compared at two time points. One month after surgery, animals were killed and the eyes were processed for histopathology. The fibrin glue-double layer AM composites had more ideal biomechanical properties. In fibrin glue-double layer AM group, the rate of epithelial healing, vascularization inhibition and corneal clarity was significantly better than the other two groups. Novel fibrin glue-double layer AMT with corneal alkali burns is more effective and useful for ocular surface reconstruction and has great potential applications.

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Acknowledgments

This study was supported by Grants from the Chongqing Science and Technology Committee (CSTC, 2008AC0086) and the First Affiliated Hospital of Chongqing Medical University (YXJJ2009-09). The authors thank Wenjun Zhou for providing technical assistance.

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    Authors and Affiliations

    1. Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

      Mingming Cai & Min Zhao

    2. Department of Ophthalmology, The Ninth People’s Hospital of Chongqing, Chongqing, 400700, China

      Mingming Cai

    3. Department of Urology, The Ninth People’s Hospital of Chongqing, Chongqing, 400700, China

      Jie Zhang

    4. Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

      Lili Guan

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    1. Mingming Cai
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    4. Min Zhao
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    Correspondence to Min Zhao.

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    Jie Zhang and Mingming Cai are common first author and contributed equally to this work.

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    Cai, M., Zhang, J., Guan, L. et al. Novel implantable composite biomaterial by fibrin glue and amniotic membrane for ocular surface reconstruction. J Mater Sci: Mater Med 26, 149 (2015). https://doi.org/10.1007/s10856-015-5491-6

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    • DOI: https://doi.org/10.1007/s10856-015-5491-6

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