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RETRACTED ARTICLE: Decellularized scaffolds containing hyaluronic acid and EGF for promoting the recovery of skin wounds

  • Tissue Engineering Constructs and Cell Substrates
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This article was retracted on 26 May 2021

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Abstract

There is no effective therapy for the treatment of deep and large area skin wounds. Decellularized scaffolds can be prepared from animal tissues and represent a promising biomaterial for investigation in tissue regeneration studies. In this study, MTT assay showed that epidermal growth factor (EGF) increased NIH3T3 cell proliferation in a bell-shaped dose response, and the maximum cell proliferation was achieved at a concentration of 25 ng/ml. Decellularized scaffolds were prepared from pig peritoneum by a series of physical and chemical treatments. Hyaluronic acid (HA) increased EGF adsorption to the scaffolds. Decellularized scaffolds containing HA sustained the release of EGF compared to no HA. Rabbits contain relatively large skin surface and are less expensive and easy to be taken care, so that a rabbit wound healing model was use in this study. Four full-thickness skin wounds were created in each rabbit for evaluation of the effects of the scaffolds on the skin regeneration. Wounds covered with scaffolds containing either 1 or 3 μg/ml EGF were significantly smaller than with vaseline oil gauzes or with scaffolds alone, and the wounds covered with scaffolds containing 1 μg/ml EGF recovered best among all four wounds. Hematoxylin-Eosin staining confirmed these results by demonstrating that significantly thicker dermis layers were also observed in the wounds covered by the decellularized scaffolds containing HA and either 1 or 3 μg/ml EGF than with vaseline oil gauzes or with scaffolds alone. In addition, the scaffolds containing HA and 1 μg/ml EGF gave thicker dermis layers than HA and 3 μg/ml EGF and showed the regeneration of skin appendages on day 28 post-transplantation. These results demonstrated that decellularized scaffolds containing HA and EGF could provide a promising way for the treatment of human skin injuries.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 30870650 and Grant No. 31171304 to Xing Wei), Research Foundation for Doctoral Discipline of Higher Education (Grant No. 20114401110007 to Xing Wei), and the Fundamental Research Funds for the Central Universities (Grant No. 21612107 to Xing Wei).

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    Authors and Affiliations

    1. Key Lab for Genetic Medicine of Guangdong Province, National Engineering Research Center of Genetic Medicine, Institute of Biomedicine, Jinan University, Guangzhou, 510632, Guangdong, China

      Zhengzheng Wu, Hongdou Fang, Zhongchun Su & Xing Wei

    2. Multidisciplinary Research Center, College of Science, Shantou University, Shantou, 515063, Guangdong, China

      Yan Tang

    3. Grandhope Biotech Co., Ltd., Building D, #408, Guangzhou International Business Incubator, Guangzhou Science Park, Guangzhou, 510663, Guangdong, China

      Bin Xu, Yongliang Lin & Peng Zhang

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    1. Zhengzheng Wu
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    Correspondence to Xing Wei.

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    Zhengzheng Wu, Yan Tang, Hongdou Fang and Zhongchun Su contributed equally to this paper.

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    Wu, Z., Tang, Y., Fang, H. et al. RETRACTED ARTICLE: Decellularized scaffolds containing hyaluronic acid and EGF for promoting the recovery of skin wounds. J Mater Sci: Mater Med 26, 59 (2015). https://doi.org/10.1007/s10856-014-5322-1

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    • DOI: https://doi.org/10.1007/s10856-014-5322-1

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