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Montmorillonite clay based polyurethane nanocomposite as substrate for retinal pigment epithelial cell growth

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Abstract

The subretinal transplantation of retinal pigment epithelial cells (RPE cells) grown on polymeric supports may have interest in retinal diseases affecting RPE cells. In this study, montmorillonite based polyurethane nanocomposite (PU-NC) was investigated as substrate for human RPE cell growth (ARPE-19 cells). The ARPE-19 cells were seeded on the PU-NC, and cell viability, proliferation and differentiation were investigated. The results indicated that ARPE-19 cells attached, proliferated onto the PU-NC, and expressed occludin. The in vivo ocular biocompatibility of the PU-NC was assessed by using the HET-CAM; and through its implantation under the retina. The direct application of the nanocomposite onto the CAM did not compromise the vascular tissue in the CAM surface, suggesting no ocular irritancy of the PU-NC film. The nanocomposite did not elicit any inflammatory response when implanted into the eye of rats. The PU-NC may have potential application as a substrate for RPE cell transplantation.

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Acknowledgments

The authors would like to acknowledge financial support from the following institutions: CAPES/MEC (Brazil), CNPq/MCT (Brazil) and FAPEMIG (Minas Gerais—Brazil).

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Authors and Affiliations

  1. School of Pharmacy, Federal University of São João Del Rei, Av. Sebastião Gonçalves Coelho, 400, Chanadour, Divinópolis, Minas Gerais, 35500-296, Brazil

    Gisele Rodrigues Da Silva

  2. School of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Armando Da Silva-Cunha Jr., Lorena Carla Vieira & Lívia Mara Silva

  3. Department of Materials, Technologies and Processes, Minas Gerais State University, Belo Horizonte, Minas Gerais, Brazil

    Eliane Ayres

  4. Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Rodrigo Lambert Oréfice

  5. Ezequiel Dias Foundation, Belo Horizonte, Minas Gerais, Brazil

    Silvia Ligório Fialho

  6. INSERM UMR872, Physiopathology of ocular diseases: Therapeutic innovations, Institut des Cordeliers, Paris, France

    Juliana Barbosa Saliba & Francine Behar-Cohen

  7. Fondation Ophtalmologique Adolphe de Rothschild, Paris, France

    Francine Behar-Cohen

  8. Hotel Dieu University Hospital, Université René Descartes, Place de Parvis Notre Dame, 1, 75004, Paris, France

    Francine Behar-Cohen

Authors
  1. Gisele Rodrigues Da Silva
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  2. Armando Da Silva-Cunha Jr.
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  3. Lorena Carla Vieira
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  4. Lívia Mara Silva
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  5. Eliane Ayres
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  6. Rodrigo Lambert Oréfice
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  7. Silvia Ligório Fialho
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  8. Juliana Barbosa Saliba
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  9. Francine Behar-Cohen
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Corresponding author

Correspondence to Gisele Rodrigues Da Silva.

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Da Silva, G.R., Da Silva-Cunha, A., Vieira, L.C. et al. Montmorillonite clay based polyurethane nanocomposite as substrate for retinal pigment epithelial cell growth. J Mater Sci: Mater Med 24, 1309–1317 (2013). https://doi.org/10.1007/s10856-013-4885-6

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  • DOI: https://doi.org/10.1007/s10856-013-4885-6

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