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Small-diameter tissue engineered vascular graft made of electrospun PCL/lecithin blend

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Abstract

In this study, natural lecithin was incorporated into cholesterol-poly(ε-caprolactone) (Chol-PCL) by solution blending in order to modify the performance of the hydrophobic and bio-inert PCL. The fibrous Chol-PCL/lecithin membranes were fabricated by electrospinning, and the surface morphology and properties were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, static water contact angle, and mechanical tensile testing. The blood compatibility of the scaffolds was evaluated by in vitro hemolysis assay. The cytocompatibility of the scaffolds was investigated by cell adhesion and proliferation using bone-marrow mesenchymal stem cells (MSCs). Subcutaneous implantation was also performed to evaluate the in vivo inflammatory reaction. The tubular tissue-engineered vascular graft (TEVG) was further constructed by rolling cell sheet comprising fibrous membrane and MSCs. Furthermore, endothelial cells (ECs) were seeded onto the lumen of the graft with the aim to form vascular endothelium. The preliminary results indicate that electrospun Chol-PCL/lecithin scaffolds show improved hemocompatibility and cytocompatibility compared with neat Chol-PCL, and combining the Chol-PCL/lecithin fibrous scaffold with MSCs and ECs with well controlled distribution is a promising strategy for constructing TEVGs.

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Acknowledgments

The work was financially supported by NSFC (Nos. 50830104 and 81000680), Research Fund for the Doctoral Program of Higher Education of China (No. 20100031120021), and Science & Technology Project of Tianjin of China (No. 12JCQNJC09300).

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Authors and Affiliations

  1. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China

    Kai Wang, Qiang Zhao & Deling Kong

  2. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China

    Min Zhang, Kai Wang, Zhexiang Wang, Bin Xing, Qiang Zhao & Deling Kong

Authors
  1. Min Zhang
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  2. Kai Wang
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  3. Zhexiang Wang
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  4. Bin Xing
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  5. Qiang Zhao
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  6. Deling Kong
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Corresponding authors

Correspondence to Qiang Zhao or Deling Kong.

Additional information

Min Zhang and Kai Wang contributed equally to this study.

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Zhang, M., Wang, K., Wang, Z. et al. Small-diameter tissue engineered vascular graft made of electrospun PCL/lecithin blend. J Mater Sci: Mater Med 23, 2639–2648 (2012). https://doi.org/10.1007/s10856-012-4721-4

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  • DOI: https://doi.org/10.1007/s10856-012-4721-4

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