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Autologous urothelial cells transplantation onto a prefabricated capsular stent for tissue engineered ureteral reconstruction

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Abstract

In this study, we have fabricated an artificial ureter by transplantation of in vitro-expanded urothelial cells onto an in vivo-prefabricated capsular stent using tissue engineering methods. Spiral poly (l-lactic acid) (PLLA) stents were transplanted into the subcutaneous of Wistar rats for a period of 1, 2 or 3 weeks to induce the formation of connective tissue capsules on their surfaces. The capsular PLLA stents were then decellularized and further recellularized with bladder epithelial cells to fabricate artificial ureters. The results showed that the entrapped cells in all capsules remained continuously proliferation and lined up in continuous layers. In addition, the urothelial cells on the capsular stents with an embedding period of 2 or 3 weeks showed higher proliferative viability compared with the cells on the stents with an embedding time of 1 week (P < 0.05). The results of the study indicated that the prefabricated capsular stents could serve as alternative cell carriers for tissue engineered ureters, especially with embedding time from 2 to 3 weeks.

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Acknowledgments

This study was supported by National Natural Science Foundation of China (No. 81070555), Beijing Natural Science Foundation (No. 2092029) and the Major Project of Clinical High and New Technology of Army hospital (No. 413DG63J).

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Authors and Affiliations

  1. Department of Urology, PLA General Hospital, Military Postgraduate Medical College, No.28 Fuxing Road, Haidian District, Beijing, 100853, China

    Yongde Xu, Weijun Fu, Jianguo Shi, Haisong Tan & Xu Zhang

  2. Organ Transplant Center, 309 Hospital of PLA, No.17 Heishanhu Road, Haidian District, Beijing, 100091, China

    Gang Li

  3. Biomaterials Lab, School of Materials Science and Engineering, Tsinghua University, Haidian District, Beijing, 100084, China

    Kun Hu & Fuzhai Cui

  4. Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, No.27 Taiping Road, Beijing, 100850, China

    Qiuxia Lin

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  1. Yongde Xu
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Correspondence to Weijun Fu.

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Yongde Xu and Weijun Fu are Co-1st authors.

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Xu, Y., Fu, W., Li, G. et al. Autologous urothelial cells transplantation onto a prefabricated capsular stent for tissue engineered ureteral reconstruction. J Mater Sci: Mater Med 23, 1119–1128 (2012). https://doi.org/10.1007/s10856-012-4583-9

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