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Crosslinking of gelatin-based drug carriers by genipin induces changes in drug kinetic profiles in vitro

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Abstract

Hydrogels are extensively studied as carrier matrices for the controlled release of bioactive molecules. The aim of this study was to design gelatin-based hydrogels crosslinked with genipin and study the impact of crosslinking temperature (5, 15 or 25°C) on gel strength, microstructure, cytocompatibility, swelling and drug release. Gels crosslinked at 25°C exhibited the highest Flory–Rehner crosslink density, lowest swelling ratio and the slowest release of indomethacin (Idn, model anti-inflammatory drug). Diffusional exponents (n) indicated non-Fickian swelling kinetics while drug transport was anomalous. Hydrogel biocompatibility, in vitro cell viability, cell cycle experiments with AH-927 and HaCaT cell lines indicated normal cell proliferation without any effect on cell cycle. Overall, these results substantiated the use of genipin-crosslinked hydrogels as a viable carrier matrix for drug release applications.

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Acknowledgments

Funding from the Natural Science and Engineering Research Council of Canada (NSERC) is acknowledged. Author Thakur acknowledges Sutapa Mukherjee, Joydip Kundu, Pallab Dutta, Shubhadeep Banerjee and Sanat Dey of Indian Institute of Technology Kharagpur and Muhammad Ali Naqvi, Renuka Gupta, Roomana Aafaqi of Ryerson University for their technical assistance. He is also thankful to Dr. Mahitosh Mandal for access to cell culture facilities.

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Authors and Affiliations

  1. School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India

    Goutam Thakur, Analava Mitra & Siddik Sarkar

  2. Department of Chemistry and Biology, Ryerson University, 350 Victoria St., Toronto, ON, M5B 2K3, Canada

    Dérick Rousseau

  3. Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India

    Amit Basak

  4. Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Rourkela, Orissa, 769008, India

    Kunal Pal

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  1. Goutam Thakur
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Correspondence to Dérick Rousseau.

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Thakur, G., Mitra, A., Rousseau, D. et al. Crosslinking of gelatin-based drug carriers by genipin induces changes in drug kinetic profiles in vitro. J Mater Sci: Mater Med 22, 115–123 (2011). https://doi.org/10.1007/s10856-010-4185-3

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  • DOI: https://doi.org/10.1007/s10856-010-4185-3

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