Abstract
Hydroxyapatite (HA)-type I collagen (Col) composite is a tissue-engineered bone graft which can act as a carrier or a template structure for cells or any other agents. In this paper, the effect of Col ratio on the scaffold structure and composition was analyzed. Scaffolds composed by HA/Col with different weight ratios (80:20; 50:50; 20:80, and 10:90) were produced by the precipitation method at pH 8–9, 37°C and 6 h of ripening. Using X-ray diffraction data, the Rietveld structure refinement showed that the size of HA crystals along the c-axis direction (002) decreases significantly in the presence of Col. Thus, the HA crystal shape turned from needle-like in pure HA, into spherical, in the 10:90 composite due to Col fibrillogenesis. The homogeneity of the composite was significantly dependent on the amount of Col in it. HA/Col 20/80 composite presented HA particles in a more homogenous way. Such a biocomposite was successfully produced in a rapid way and it is potentially useful for both small tissue repairs and engineering.
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Acknowledgements
The authors thank Dr. Virginia C. A. Martins for providing collagen as well as and helpful suggestions. This work was supported by renowned Brazilian agencies such as CNPq, CAPES and FAPERJ.
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Sena, L.A., Caraballo, M.M., Rossi, A.M. et al. Synthesis and characterization of biocomposites with different hydroxyapatite–collagen ratios. J Mater Sci: Mater Med 20, 2395–2400 (2009). https://doi.org/10.1007/s10856-009-3813-2
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DOI: https://doi.org/10.1007/s10856-009-3813-2