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The preparation, cytocompatibility, and in vitro biodegradation study of pure β-TCP on magnesium

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Abstract

Biodegradable and bioactive β-tricalcium phosphate (β-TCP) coatings were prepared on magnesium (Mg) in order to improve its biocompatibility by a chemical method. The tensile bonding strength of β-TCP coating and Mg substrate was measured by the standard adhesion test (ISO 13779-4). And the cytocompatibility of β-TCP coated Mg was studied by using human osteoblast-like MG63 cells. It was found that the MG63 cells could grow well on the surface of β-TCP coated Mg and the cell viability on β-TCP coated Mg was above 80% during the cocultivation of MG63 cells and β-TCP coated Mg for 10 days, indicating no cytotoxicity. It was concluded that the β-TCP coated Mg had good cytocompatibility. The degradation of Mg substrate with β-TCP coating in vitro was studied in detail by XRD, EDX, SEM, and ICP. The results showed that a bone-like apatite continually formed on the surface of the sample with the degradation of both Mg substrate and β-TCP coating in Hank’s solution (a simulated body fluid). The biodegradation mechanism was preliminarily analyzed in the paper.

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Acknowledgments

This work was partly supported by a fund from Chinese Academy of Sciences, Applied Research of Bioactive Bone Implanted Materials (No. KGCX2-YW-207).

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Authors and Affiliations

  1. Institute of Metal Research, Chinese Academy of Sciences, Shenyang, 110016, People’s Republic of China

    F. Geng, L. L. Tan & K. Yang

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    F. Geng

  3. Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, 110016, People’s Republic of China

    X. X. Jin & J. Y. Yang

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  1. F. Geng
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Correspondence to K. Yang.

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Geng, F., Tan, L.L., Jin, X.X. et al. The preparation, cytocompatibility, and in vitro biodegradation study of pure β-TCP on magnesium. J Mater Sci: Mater Med 20, 1149–1157 (2009). https://doi.org/10.1007/s10856-008-3669-x

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