Abstract
Protein adsorption onto solid surfaces is a complex process playing an important role in biological systems, being crucial to maintain the three-dimensional conformation during molecules interaction. Polymers hybrid network could be used to perform immunoassays with the required high level specificity. We have developed a novel route for incorporating recombinant protein from Bovine Herpesvirus into a network using as template Poly(vinyl alcohol) chemically tailored with five organosilanes followed by FTIR spectroscopy characterization. Protein expressed in E. coli was incorporated into hybrid network; all the PVA-hybrids interacted with this biomolecule and FTIR vibrational bands have endorsed the results. PVA hybrids modified with 3-(triethoxysilyl) propylisocyanate-TESPI presented the best result in the immunoassay, enhancing the sera specificity in comparison with the commercial microplate. We have successfully developed organic–inorganic hybrid based on PVA-silane to be used in serological tests with recombinant protein as a promising tool for the development and production new diagnostic assays.
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Acknowledgments
The authors acknowledge CNPq/FAPEMIG/CAPES for financial support on this Project. HS Mansur, EF Barbosa-Stancioli and G. I. Andrade received research fellowship from CNPq, and R. M. Palhares and A. A. P. Mansur received fellowship from FAPEMIG.
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Mansur, H.S., Palhares, R.M., Andrade, G.I. et al. Improvement of viral recombinant protein-based immunoassays using nanostructured hybrids as solid support. J Mater Sci: Mater Med 20, 513–519 (2009). https://doi.org/10.1007/s10856-008-3606-z
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DOI: https://doi.org/10.1007/s10856-008-3606-z