Abstract
Matured osteoblasts were proved to be located in the bone formation accelerated by induced large surface charges on the electrically polarized hydroxyapatite (HA) ceramics regardless of the charge polarities, whereas the spatial cell distribution patterns were different. Polarized HA ceramic plates with an average electric charge of 3.9 µCcm−2 were implanted in widely spaced defects of canine femora for 3 and 7 days. The osteoblasts were identified by immunochemical detections of osteocalcin and osteopontin. Expressions of osteocalcin and osteopontin were detected throughout the gaps between the implanted HA plates and the cut cortical bone surfaces, especially in the vicinities of the cut cortical bone surfaces and the osteoids regardless of the polarity of the induced charges. Additionally, the newly formed bone tissue that directly bonded to the negatively charged HA surfaces was lined by an osteoblast layer. As soon as 7 days after the implantation, the presence of well-developed osteoblasts suggested that the electrostatic force of the HA ceramics had conditioned the field in the biointerface zone of the polarized HA surfaces.
Similar content being viewed by others
References
E. Fukada, I. Yasuda, J. Phys. Soc. Jpn. 12, 1158 (1957). doi:10.1143/JPSJ.12.1158
C.A.L. Bassett, R.O. Becker, Science 137, 1063 (1962). doi:10.1126/science.137.3535.1063
C.A.L. Bassett, R.J. Pawluk, R.O. Becker, Nature 204, 652 (1964). doi:10.1038/204652a0
M. Krukowski, R.A. Shively, P. Osdoby, B.L. Eppley, J. Oral Maxillofac. Surg. 48, 468 (1990). doi:10.1016/0278-2391(90)90233-R
M. Krukowski, D.J. Simmons, A. Summerfield, P. Osdoby, J. Bone Miner. Res. 3(2), 165 (1988)
N. Hamamoto, Y. Hamamoto, T. Nakajima, H. Ozawa, Arch. Oral Biol. 40(2), 97 (1995). doi:10.1016/0003-9969(94)00153-3
S.A. Makohliso, R.F. Valentini, P. Aebischer, J. Biomed. Mater. Res. 27, 1075 (1993). doi:10.1002/jbm.820270813
K. Yamashita, N. Oikawa, T. Umegaki, Chem. Mater. 8, 2697 (1996). doi:10.1021/cm9602858
S. Nakamura, H. Takeda, K. Yamashita, J. Appl. Phys. 89(10), 5386 (2001). doi:10.1063/1.1357783
K. Yamashita, H. Owada, H. Nakagawa, T. Umegaki, T. Kanazawa, J. Am. Ceram. Soc. 65(8), 590 (1986). doi:10.1111/j.1151-2916.1986.tb04813.x
K. Yamashita, H. Owada, T. Umegaki, T. Kanazawa, T. Futagami, Solid State Ionics 28–30, 660 (1988). doi:10.1016/S0167-2738(88)80121-8
T. Kobayashi, S. Nakamura, K. Yamashita, J. Biomed. Mater. Res. 57(4), 477 (2001). doi :10.1002/1097-4636(20011215)57:4<477::AID-JBM1193>3.0.CO;2-5
S. Nakamura, T. Kobayashi, K. Yamashita, J. Biomed. Mater. Res. 61(4), 593 (2002). doi:10.1002/jbm.10224
S. Nakamura, T. Kobayashi, K. Yamashita, J. Biomed. Mater. Res. 68A(1), 90 (2004). doi:10.1002/jbm.a.10124
S. Itoh, S. Nakamura, T. Kobayashi, K. Shinomiya, K. Yamashita, Calcif. Tissue Int. 78(3), 133 (2006). doi:10.1007/s00223-005-0213-6
K. Yamashita, K. Kitagawa, T. Umegaki, J. Am. Ceram. Soc. 78(5), 1191 (1995). doi:10.1111/j.1151-2916.1995.tb08468.x
J.A. Cobos, J. Yang, R. Zhang, M. Krukowski, D.J. Simmons, J. Biomed. Mater. Res. 39, 458 (1998). doi :10.1002/(SICI)1097-4636(19980305)39:3<458::AID-JBM16>3.0.CO;2-6
A. Chierico, R. Valentini, Z. Majzoub, A. Piatelli, A. Scarano, L. Okun et al., Clin. Oral Implants Res. 10, 415 (1999). doi:10.1034/j.1600-0501.1999.100508.x
M. Nakamura, Y. Sekijima, S. Nakamura, T. Kobayashi, K. Niwa, K. Yamashita, J. Biomed. Mater. Res. 79A(3), 627 (2007)
R.H. Chrisenson, Clin. Biochem. 30(8), 573 (1997). doi:10.1016/S0009-9120(97)00113-6
K. Yoon, R. Buenaga, G.A. Rodan, Biochem. Biophys. Res. Commun. 148(3), 1129 (1987). doi:10.1016/S0006-291X(87)80250-4
K. Nose, H. Saito, T. Kuroki, Cell Growth Differ. 1(11), 511 (1990)
K. Yamashita, S. Nakamura, J. Ceram. Soc. Jpn. 113(1), 1 (2005). doi:10.2109/jcersj.113.1
Acknowledgments
The authors appreciate the help of Prof. Shimokawa of Tokyo Medical and Dental University with immunochemical staining. Funding sources: Grants-in-Aid from the Japan Society for the Promotion of Science (#15360338). Grant for Development of Advanced Medical Technology from the Ministry of Education, Science, Sports and Culture of Japan.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Nakamura, S., Kobayashi, T., Nakamura, M. et al. Enhanced in vivo responses of osteoblasts in electrostatically activated zones by hydroxyapatite electrets. J Mater Sci: Mater Med 20, 99–103 (2009). https://doi.org/10.1007/s10856-008-3546-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10856-008-3546-7