Skip to main content

Advertisement

SpringerLink
Log in

Biological response of human bone marrow stromal cells to sandblasted titanium nitride-coated implant surfaces

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Titanium nitride (TiN) coating has been proposed as an adjunctive surface treatment aimed to increase the physico-mechanical and aesthetic properties of dental implants. In this study we investigated the biological response of primary human bone marrow stromal cells (BMSC) to TiN-coated sandblasted (TiN-SB) compared to uncoated sandblasted (SB) surfaces. SB and TiN-SB disks were qualitatively and quantitatively analyzed by atomic force microscopy. BMSC were obtained from healthy donors and their adhesion and proliferation on the titanium disks were evaluated by scanning electron microscopy and viability assay. The osteoblastic differentiation, in terms of alkaline phosphatase activity, osteocalcin synthesis, and extracellular mineralization, was assessed by specific immunoenzymatic or spectrophotometric assays. No difference (P > 0.05) between TiN-SB and SB disks was found in terms of any of the investigated parameters. TiN-coating showed to maintain the topographical characteristics of sandblasted titanium surfaces and their biological affinity toward bone precursors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. D. Buser, R. Mericske-Stern, J.P. Bernard, A. Behneke, N. Behneke, H.P. Hirt, U.C. Belser, N.P. Lang, Clin. Oral Implants Res. 8, 161 (1997)

    Article  CAS  Google Scholar 

  2. N. Ferrigno, M. Laureti, S. Fanali, G. Grippaudo, Clin. Oral Implants Res. 13, 260 (2002)

    Article  Google Scholar 

  3. J.A. Ekelund, L.W. Lindquist, G.E. Carlsson, T. Jemt, Int. J. Prosthodont. 16, 602 (2003)

    Google Scholar 

  4. L. Rasmusson, J. Roos, H. Bystedt, Clin. Implant Dent. Relat. Res. 7, 36 (2005)

    Article  Google Scholar 

  5. L.F. Cooper, J. Prosthet. Dent. 84, 522 (2000)

    Article  CAS  Google Scholar 

  6. T. Albrektsson, A. Wennerberg, Int. J. Prosthodont. 17, 536 (2004)

    Google Scholar 

  7. T. Albrektsson, A. Wennerberg, Int. J. Prosthodont. 17, 544 (2004)

    Google Scholar 

  8. L.F. Cooper, T. Masuda, S.W. Whitson, P. Yliheikkila, D.A. Felton, Int. J. Oral Maxillofac. Implants 14, 37 (1999)

    CAS  Google Scholar 

  9. L. Marinucci, S. Balloni, E. Becchetti, S. Belcastro, M. Guerra, M. Calvitti, C. Lilli, E.M. Calvi, P. Locci, Int. J. Oral Maxillofac. Implants. 21, 719 (2006)

    Google Scholar 

  10. K. Mustafa, A. Wennerberg, J. Wroblewski, K. Hultenby, B.S. Lopez, K. Arvidson, Clin. Oral Implants Res. 12, 515 (2001)

    Article  CAS  Google Scholar 

  11. C. Masaki, G.B. Schneider, R. Zaharias, D. Seabold, C. Stanford, Clin. Oral Implants Res. 16, 650 (2005)

    Article  Google Scholar 

  12. K. Gotfredsen, T. Berglundh, J. Lindhe, Clin. Implant Dent. Relat. Res. 2, 120 (2000)

    Article  CAS  Google Scholar 

  13. S.A. Cho, K.T. Park, Biomaterials 24, 3611 (2003)

    Article  CAS  Google Scholar 

  14. G. Cordioli, Z. Majzoub, A. Piattelli, A. Scarano, Int. J. Oral Maxillofac. Implants 15, 668 (2000)

    CAS  Google Scholar 

  15. R. Mengel, C.E. Buns, C. Mengel, L. Flores-De-Jacoby, Int. J. Oral Maxillofac. Implants 13, 91 (1998)

    CAS  Google Scholar 

  16. L. Ramaglia, A.E. Di Lauro, F. Morgese, A. Squillace, Implant Dent. 15, 77 (2006)

    Article  Google Scholar 

  17. H. Kawashima, S. Sato, M. Kishida, H. Yagi, K. Matsumoto, K. Ito, J. Periodontol. 78, 1689 (2007)

    Article  CAS  Google Scholar 

  18. R. Mengel, C. Meer, L. Flores-De-Jacoby, Int. J. Oral Maxillofac Implants 19, 232 (2004)

    Google Scholar 

  19. T. Sawase, K. Yoshida, Y. Taira, K. Kamada, M. Atsuta, K. Baba, J. Oral Rehabil. 32, 151 (2005)

    Article  CAS  Google Scholar 

  20. B. Grössner-Schreiber, M. Griepentrog, I. Haustein, W.D. Müller, K.P. Lange, H. Briedigkeit, U.B. Göbel, Clin. Oral Implants Res. 12, 543 (2001)

    Article  Google Scholar 

  21. A. Scarano, M. Piattelli, G. Vrespa, S. Caputi, A. Piattelli, J Oral Implantol. 29, 80 (2003)

    Article  Google Scholar 

  22. ISO 4287:1997 Geometrical Product Specifications (GPS)—Surface texture: Profile method—Terms, Definitions and Surface texture parameters

  23. A. Oliva, I. Passaro, R. Di Pasquale, A. Di Feo, M. Criscuolo, V. Zappia, F. Della Ragione, S. D’amato, M. Annunziata, L. Guida, Int. J. Immunopathol. Pharmacol. 18, 47 (2005)

    CAS  Google Scholar 

  24. L. Le Guéhennec, A. Soueidan, P. Layrolle, Y. Amouriq, Dent. Mater. 23, 844 (2007)

    Article  Google Scholar 

  25. M. Quirynen, M. Abarca, N.V.A.N. Assche, M. Nevins, D.V.A.N. Steenberghe, J. Clin. Periodontol. 34, 805 (2007)

    Article  Google Scholar 

  26. W. Teughels, N.V.A.N. Assche, I. Sliepen, M. Quirynen, Clin. Oral. Implants Res. 17, 68 (2006)

    Article  Google Scholar 

  27. W.D. Sproul, Surf. Coat. Tech. 81, 1 (1996)

    Article  CAS  Google Scholar 

  28. A. Scarano, M. Piattelli, G. Vrespa, G. Petrone, G. Iezzi, A. Piattelli, Clin. Implant Dent. Relat. Res. 5, 103 (2003)

    Article  Google Scholar 

  29. B. Groessner-Schreiber, A. Neubert, W.D. Müller, M. Hopp, M. Griepentrog, K.P. Lange, J. Biomed. Mater. Res. A 64, 591 (2003)

    Article  Google Scholar 

  30. W.C. Clem, V.V. Konovalov, S. Chowdhury, Y.K. Vohra, S.A. Catledge, S.L. Bellis, J. Biomed. Mater. Res. A 76, 279 (2006)

    Google Scholar 

  31. M. Manso-Silvan, J.M. Martínez-Duart, S. Ogueta, P. García-Ruiz, J. Pérez-Rigueiro, J. Mater. Sci. Mater. Med. 13, 289 (2002)

    Article  CAS  Google Scholar 

  32. K. Bordji, J.Y. Jouzeau, D. Mainard, E. Payan, P. Netter, K.T. Rie, T. Stucky, M. Hage-Ali, Biomaterials 17, 929 (1996)

    Article  CAS  Google Scholar 

  33. J.B. Lian, G.S. Stein, Crit. Rev. Oral Biol. Med. 3, 269 (1992)

    CAS  Google Scholar 

  34. P. Ducy, C. Desbois, B. Boyce, G. Pinero, B. Story, C. Dunstan, E. Smith, J. Bonadio, S. Goldstein, C. Gundberg, A. Bradley, G. Karsenty, Nature 382, 448 (1996)

    Article  CAS  Google Scholar 

  35. S. Harada, G.A. Rodan, Nature 423, 349 (2003)

    Article  CAS  Google Scholar 

  36. N.K. Lee, H. Sowa, E. Hinoi, M. Ferron, J.D. Ahn, C. Confavreux, R. Dacquin, P.J. Mee, M.D. Mckee, D.Y. Jung, Z. Zhang, J.K. Kim, F. Mauvais-Jarvis, P. Ducy, G. Karsenty, Cell 130, 456 (2007)

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by Research Grant from the Second University of Naples, Naples, Italy. The authors would like to thank P.H.I. S.r.l. (San Vittore Olona, Milano, Italy) for kindly providing the titanium specimens used in the experimentation.

Author information

Authors and Affiliations

  1. Department of Odontostomatological, Orthodontic and Surgical Disciplines, Second University of Naples, Via L. De Crecchio, 6, 80138, Naples, Italy

    Marco Annunziata, Luigi Guida & Letizia Perillo

  2. Department of Materials and Production Engineering (DIMP), School of Engineering, University of Naples “Federico II”, Piazz.le Tecchio, 80, 80125, Naples, Italy

    Raffaella Aversa

  3. Department of Biochemistry and Biophysics “F. Cedrangolo”, Second University of Naples, Via L. De Crecchio, 7, 80138, Naples, Italy

    Irene Passaro & Adriana Oliva

Authors
  1. Marco Annunziata
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luigi Guida
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Letizia Perillo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Raffaella Aversa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Irene Passaro
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Adriana Oliva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Luigi Guida.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Annunziata, M., Guida, L., Perillo, L. et al. Biological response of human bone marrow stromal cells to sandblasted titanium nitride-coated implant surfaces. J Mater Sci: Mater Med 19, 3585–3591 (2008). https://doi.org/10.1007/s10856-008-3514-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-008-3514-2

Keywords

Search

Navigation