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Histological study of surface modified three dimensional poly (d, l-lactic acid) scaffolds with chitosan in vivo

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Abstract

Biocompatibility and tissue regenerating capacity are essential for biomaterials that used in tissue engineering. The aim of this study was to histologically assess the tissue reactions and bone conductivities of surface modified three dimensional (3-D) poly (d, l-lactic acid) (PDLLA) scaffolds, which were coated with chitosan via a physical entrapment method. The native PDLLA scaffold was prepared via thermally induced phrase separation technique and was characterized by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). Osteocalcin assay, a method to evaluate the bone formation potential, has shown that the osteocalcin production in chitosan-modified 3-D PDLLA scaffold group was significantly higher (p < 0.05) than that of in control. The tissue reactions and bone conductivities between surface modified PDLLA and native PDLLA scaffolds were evaluated using a rabbit radialis defect model in vivo and compared at different implantation intervals (2, 4, 8 and 12 weeks). The histological results have shown a higher bone formation potential and better biocompatibility of chitosan-modified 3-D PDLLA scaffolds as compared with the control group scaffolds.

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Acknowledgement

This research was performed with support from the National Basic Science Research and Development Grants (973) via grant G199954035 and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Chong Qing University.

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Authors and Affiliations

  1. College of Bioengineering, Chong Qing University, Chong Qing, 400044, P.R. China

    Kaiyong Cai

  2. Research Institute of Polymeric Materials, Tianjin University, Tianjin, 300072, P.R. China

    Kangde Yao

  3. The Medical Center, Si Chuan University, Chengdu, 610041, P.R. China

    Zhiming Yang, Yanlong Qu & Xiuqiong Li

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  1. Kaiyong Cai
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  2. Kangde Yao
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  3. Zhiming Yang
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Correspondence to Kaiyong Cai.

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Cai, K., Yao, K., Yang, Z. et al. Histological study of surface modified three dimensional poly (d, l-lactic acid) scaffolds with chitosan in vivo. J Mater Sci: Mater Med 18, 2017–2024 (2007). https://doi.org/10.1007/s10856-007-3151-1

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