Skip to main content
SpringerLink
Log in

Osteoprogenitor response to low-adhesion nanotopographies originally fabricated by electron beam lithography

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

It is considered that cells can use filopodia, or microspikes, to locate sites suitable for adhesion. This has been investigated using a number of mature cell types, but, to our knowledge, not progenitor cells. Chemical and topographical cues on the underlying substrate are a useful tool for producing defined features for cells to respond to. In this study, arrays of nanopits with different symmetries (square or hexagonal arrays with 120 nm diameters, 300 nm center–centre spacings) and osteoprogenitor cells were considered. The pits were fabricated by ultra-high precision electron-beam lithography and then reproduced in polycarbonate by injection moulding with a nickel stamp. Using scanning electron and fluorescence microscopies, the initial interactions of the cells via filopodia have been observed, as have subsequent adhesion and cytoskeletal formation. The results showed increased filopodia interaction with the surrounding nanoarchitecture leading to a decrease in cell spreading, focal adhesion formation and cytoskeletal organisation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. A. S. G. CURTIS and M. VARDE, J. Natl. Cancer Res. Inst. 33 (1964) 15.

    CAS  Google Scholar 

  2. S. BRITLAND, H. MORGAN, B. WOJCIAK-STOTHARD, M. RIEHLE, A. CURTIS and C. WILKINSON, Exp. Cell Res. 228 (1996) 313.

    Article  CAS  Google Scholar 

  3. B. WOJCIAK-STOTHARD, A. CURTIS, W. MONAGHAN, K. MACDONALD and C. WILKINSON, Exp. Cell. Res. 223 (1996) 426.

    Article  CAS  Google Scholar 

  4. B. WOJCIAK-STOTHARD, A. S. G. CURTIS, W. MONAGHAN, M. MCGRATH, I. SOMMER and C. D. W. WILKINSON, Cell Motil. Cytoskeleton 31 (1995) 147.

    Article  CAS  Google Scholar 

  5. P. CLARK, P. CONNOLLY, A. S. CURTIS, J. A. DOW and C. D. WILKINSON, Development 99 (1987) 439.

    CAS  Google Scholar 

  6. P. CLARK, P. CONNOLLY, A. S. CURTIS, J. A. DOW and C. D. WILKINSON, Development 108 (1990) 635.

    CAS  Google Scholar 

  7. M. J. DALBY, M. O. RIEHLE, S. J. YARWOOD, C. D. WILKINSON and A. S. CURTIS, Exp. Cell. Res. 284 (2003) 274.

    Article  CAS  Google Scholar 

  8. B. KASEMO and J. LAUSMAA, Int. J. Oral Maxillofac. Implants 3 (1988) 247.

    CAS  Google Scholar 

  9. B. KASEMO and J. LAUSMAA, Environ. Health Perspect. 102(Suppl 5) (1994) 41.

    Article  Google Scholar 

  10. A. D. BERSHADSKY, N. Q. BALABAN and B. GEIGER, Annu. Rev. Cell Dev. Biol. 19 (2003) 677.

    Article  CAS  Google Scholar 

  11. K. BURRIDGE and M. CHRZANOWSKA-WODNICKA, Annu. Rev. Cell Dev. Biol. 12 (1996) 463.

    Article  CAS  Google Scholar 

  12. M. J. DALBY, M. O. RIEHLE, H. JOHNSTONE, S. AFFROSSMAN and A. S. CURTIS, Cell Biol. Int. 28 (2004) 229.

    Article  CAS  Google Scholar 

  13. M. J. DALBY, N. GADEGAARD, M. O. RIEHLE, C. D. WILKINSON and A. S. CURTIS, Int. J. Biochem. Cell Biol. 36 (2004) 2015.

    Article  CAS  Google Scholar 

  14. J. HUANG, L. DI SILVIO, M. WANG, K. E. TANNER and W. BONFIELD, J. Mater. Sci. Mater. Med. 8 (1997) 775.

    Article  CAS  Google Scholar 

  15. M. J. DALBY, L. DI SILVIO, E. J. HARPER and W. BONFIELD, Biomaterials 23 (2002) 569.

    Article  CAS  Google Scholar 

  16. J. HUANG, L. DI SILVIO, M. WANG, I. REHMAN, C. OHTSUKI and W. BONFIELD, J. Mater. Sci. Mater. Med. 8 (1997) 809.

    Article  CAS  Google Scholar 

  17. M. J. DALBY, D. PASQUI and S. AFFROSSMAN, IEE Proc. Nanobiotechnol. 151 (2004) 53.

    Article  CAS  Google Scholar 

  18. M. J. DALBY, M. O. RIEHLE, D. S. SUTHERLAND, H. AGHELI and A. S. CURTIS, Biomaterials 25 (2004) 5415.

    Article  CAS  Google Scholar 

  19. D. R. S. CUMMING, S. THOMS, S. P. BEAUMONT and J. M. R. WEAVER, Appl. Phys. Lett. 68 (1996) 322.

    Article  CAS  Google Scholar 

  20. C. VIEU, F. CARCENAC, A. PEPIN, Y. CHEN, M. MEJIAS, A. LEBIB, L. MANIN-FERLAZZO, L. COURAUD and H. LAUNOIS, Appl. Surf. Sci 164 (2000) 111.

    Article  CAS  Google Scholar 

  21. N. GADEGAARD, S. THOMS, D. S. MACINTYRE, K. MCGHEE, J. GALLAGHER, B. CASEY and C. D. W. WILKINSON, Microelectron Eng 67–68 (2003) 162.

    Article  CAS  Google Scholar 

  22. N. GADEGAARD, S. MOSLER and N. B. LARSEN, Macromol. Mater. Eng. 88 (2003) 76.

    Article  Google Scholar 

  23. H. SCHIFT, C. DAVID, M. GABRIEL, J. GOBRECHT, L. J. HEYDERMAN, W. KAISER, S. KOPPEL and L. SCANDELLA, Microelectron. Eng. 53 (2000) 1.

    Article  Google Scholar 

  24. R. O. OREFFO, S. BORD and J. T. TRIFFITT, Clin. Sci. (Lond) 94 (1998) 549.

    CAS  Google Scholar 

  25. X. B. YANG, H. I. ROACH, N. M. P. CLARK, S. M. HOWDLE, R. QUIRK, K. M. SHAKESHEFF and R. O. C. OREFFO, Bone 29 (2001) 523.

    Article  CAS  Google Scholar 

  26. A. S. CURTIS, B. CASEY, J. O. GALLAGHER, D. PASQUI, M. A. WOOD and C. D. WILKINSON, Biophys. Chem. 94 (2001) 275.

    Article  CAS  Google Scholar 

  27. J. O. GALLAGHER, K. F. MCGHEE, C. D. W. WILKINSON and M. O. RIEHLE, IEEE Trans. Nanobiosci. 1 (2002) 24.

    Article  CAS  Google Scholar 

  28. A. S. CURTIS, N. GADEGAARD, M. J. DALBY, M. O. RIEHLE, C. D. WILKINSON and G. AITCHISON, IEEE Trans. Nanobiosci. 3 (2004) 61.

    Article  CAS  Google Scholar 

  29. T. GUSTAFSON and L. WOLPERT, Exp. Cell Res. 24 (1961) 64.

    Article  CAS  Google Scholar 

  30. W. WOOD and P. MARTIN, Int. J. Biochem. Cell Biol. 34 (2002) 726.

    Article  CAS  Google Scholar 

  31. M. UEDA, Y. SAKO, T. TANAKA, P. DEVREOTES and T. YANAGIDA, Science 294 (2001) 864.

    Article  CAS  Google Scholar 

  32. M. IIJIMA and P. DEVREOTES, Cell 109 (2002) 599.

    Article  CAS  Google Scholar 

  33. A. J. KOLESKE, Sci. STKE 2003 (2003) pe20.

    Article  Google Scholar 

  34. A. RAJNICEK, S. BRITLAND and C. MCCAIG, J. Cell. Sci. 110(Pt 23) (1997) 2905.

    Google Scholar 

  35. A. RAJNICEK and C. MCCAIG, J. Cell. Sci. 110(Pt 23) (1997) 2915–2924.

    CAS  Google Scholar 

  36. E. STEPIEN, J. STANISZ and W. KOROHODA, Cell. Biol. Int. 23 (1999) 105.

    Article  CAS  Google Scholar 

  37. M. J. DALBY, M. O. RIEHLE, H. J. JOHNSTONE, S. AFFROSSMAN and A. S. CURTIS, Tissue Eng. 8 (2002) 1099.

    Article  CAS  Google Scholar 

  38. E. MARTINES, K. SEUNARINE, H. MORGAN, N. GADEGAARD, C. D. W. WILKINSON, M. O. RIEHLE, Nanoletters 5 (2005) 2097.

    CAS  Google Scholar 

Download references

Acknowledgments

MD is a BBSRC David Phillips Fellow (17/JF/20604) and is funded through this route. N. Gadegaard is a Royal Society of Edinburgh research fellow. ROCO is supported by grants from the BBSRC and EPSRC. We would like to thank John Pedersen (SDC Dandisc A/S, Denmark) for the organising and preparation of nickel shims and Stephanie Inglis for assistance with cell culture. We also thank Prof Adam Curtis and Dr Mathis Riehle for their support and discussion.

Author information

Authors and Affiliations

  1. Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK

    Andrew Hart & Matthew J. Dalby

  2. Centre for Cell Engineering, Department of Electronics and Electrical Engineering, Rankine Building, University of Glasgow, Glasgow, G12 8QQ, UK

    Nikolaj Gadegaard & Chris D. W. Wilkinson

  3. Bone and Joint Research Group, DOHAD, University of Southampton, Southampton, S016 6YD, UK

    Richard O. C. Oreffo

Authors
  1. Andrew Hart
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nikolaj Gadegaard
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chris D. W. Wilkinson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Richard O. C. Oreffo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Matthew J. Dalby
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Matthew J. Dalby.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hart, A., Gadegaard, N., Wilkinson, C.D. . et al. Osteoprogenitor response to low-adhesion nanotopographies originally fabricated by electron beam lithography. J Mater Sci: Mater Med 18, 1211–1218 (2007). https://doi.org/10.1007/s10856-007-0157-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-007-0157-7

Keywords

Search

Navigation