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Cell/surface interactions on laser micro-textured titanium-coated silicon surfaces

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Abstract

This paper examines the effects of nano-scale titanium coatings, and micro-groove/micro-grid patterns on cell/surface interactions on silicon surfaces. The nature of the cellular attachment and adhesion to the coated/uncoated micro-textured surfaces was elucidated by the visualization of the cells and relevant cytoskeletal & focal adhesion proteins through scanning electron microscopy and immunofluorescence staining. Increased cell spreading and proliferation rates are observed on surfaces with 50 nm thick Ti coatings. The micro-groove geometries have been shown to promote contact guidance, which leads to reduced scar tissue formation. In contrast, smooth surfaces result in random cell orientations and the increased possibility of scar tissue formation. Immunofluorescence cell staining experiments also reveal that the actin stress fibers are aligned along the groove dimensions, with discrete focal adhesions occurring along the ridges, within the grooves and at the ends of the cell extensions. The implications of the observed cell/surface interactions are discussed for possible applications of silicon in implantable biomedical systems.

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References

  1. J. W. GARDNER, V. K. VARADAN and O. O. AWADELKARIM, “Microsensors, MEMS, and Smart Devices” (Wiley, New York, 2002).

    Google Scholar 

  2. B. ZIAIE and K. NAJAFI, Biomed. Micr. 3(4) (2001) 285.

    Article  Google Scholar 

  3. D. LIEPMANN, in Proceedings of The 3rd Korean MEMS Conference (Seoul, 2001) p. 13.

  4. BABAK ZIAIE X. CAO, S. LAI and L. LEE, Biomed. Micr. 3(2) (2001) 109.

    Article  Google Scholar 

  5. Z. STRONG, A. WANG and C. MCCONAGHY, Biomed. Microdevices 4(2) (2002) 97.

    Article  CAS  Google Scholar 

  6. G. VOSKERICIAN, M. S. SHIVE, R. S. SHAWGO, H. VON RECUM, J. M. ANDERSON, M. J. CIMA and R. LANGER, Biomater. 24 (2003) 1959.

    Article  CAS  Google Scholar 

  7. B. D. RATNER, A. S. HOFFMAN, F. J. SCHOEN and J. E. LEMONS, “Biomaterials Science: An Introduction to Materials in Medicine” (Academic Press, San Diego, 1996).

    Google Scholar 

  8. Y. HANEIN, Y. V. PAN, B. D. RATNER, D. DENTON and K. F. BÖHRINGER, Sens. Act. B: Chem. 81(1) (2001) 49.

    Article  Google Scholar 

  9. C. MILBURN, S. MWENIFUMBO, E. KUNG and W. O. SOBOYEJO, Effects of biocompatible nano-scale titanium coatings on cell spreading and adhesion to biomems structures, to be submitted.

  10. D. M. BRUNETTE, “Titanium in Medicine: Material Science, Surface Science, Engineering, Biological Responses and Medical Applications” (Springer-Verlag, New York, 2001).

    Google Scholar 

  11. W. O. SOBOYEJO, B. NEMETSKI, S. ALLAMEH, N. MARCANTONIO, C. MERCER and J. RICCI, J. Biomed. Mater. Res. 62 (2002) 56.

    Article  CAS  Google Scholar 

  12. W. O. SOBOYEJO, C. MERCER, S. ALLAMEH, B. NEMETSKI, N. MARCANTONIO and J. L. RICCI, Key Eng. Mater. 198–199 (2001) 203.

    Google Scholar 

  13. K. ANSELME, P. LINEZ, M. BIGERELLE, D. LE MAGUER, A. LE MAGUER, P. HARDOUIN, H. F. HILDEBRAND, A. IOST and J. M. LEROY, Biomater. 21 (2000) 1567.

    Article  CAS  Google Scholar 

  14. K. ANSELME, M. BIGERELLE, B. NOEL, E. DUFRESNE, D. JUDAS, A. IOST and P. HARDOUIN Biomed. Mater. Res. 49 (2000) 155.

    Article  CAS  Google Scholar 

  15. K. ANSELME, B. NOEL and P. HARDOUN, J. Mater. Sci.: Mater. Med. 10 (1999) 815.

    Article  CAS  Google Scholar 

  16. J. L. RICCI and H. ALEXANDER, Key Eng. Mater. 198–199 (2001) 179.

    Article  Google Scholar 

  17. J. L. RICCI, J. CHARVET, S. R. FRENKEL, R. CHANG, P. NADKARNI, J. TURNER and H. ALEXANDER, “Bone response to laser microtextured surfaces”, Bone Engineering, edited by J. E. Davies, (Em2 Inc., Toronto, 2000) p. 1.

    Google Scholar 

  18. J. H-C WANG, E. S. GROOD, J. FLORER and R. WENSTRUP, J. Biomech. 33 (2000) 729.

    Article  CAS  Google Scholar 

  19. J. H.-C. WANG and E.S. GROOD, Connective Tissue Res. 41 (2000) 29.

    CAS  Google Scholar 

  20. K. D. CHESMAL and J. BLACK, J. Biomed. Mater. Res. 29 (1995) 1089.

    Article  Google Scholar 

  21. K. D. CHESMAL, C. C. CLARK, C. T. BRIGHTON and J. BLACK, J. Biomed. Mater. Res. 29 (1995) 1101.

    Article  Google Scholar 

  22. E. DEN BRABER, J. DE RUIJTER, H. SMITS, L. GINSEL, A. F. VON RECUM and J. A. JANSEN, J. Biomed. Mater. Res. 29 (1995) 511.

    Article  Google Scholar 

  23. J. L. RICCI, J. CHARVET and R. CHANG, in Proceedings of 20th Annual Meeting of the Society for Biomaterials (Boston, 1994) p. 401.

  24. P. CLARK, D. COLES and M. PECKHAM, Exp. Cell Res. 230 (1997) 275.

    Article  CAS  Google Scholar 

  25. R. F. WOOD, C. W. WHITE and R. T. YOUNG, “Semiconductors and Semimetals 23” (Academic, Orlando, 1984).

    Google Scholar 

  26. M. MOSBACHER, H. J. MUNZER, M. BERTSCH, V. DOBLER, N. CHAOUI, J. SIEGEL, R. OLTRA, D. BAUERLE, J. BONEBERG and P. LEIDERER, “Laser assisted Particle Removal from Silicon Wafers”, Particles on Surfaces: Detection, Adhesion and Removal 7, (VSP International Science Publisher, Netherlands, 2002), p. 275.

    Google Scholar 

  27. A. G. CULLIS, Reports Prog. Phys 48 (1985) 1155.

    Article  CAS  Google Scholar 

  28. S. VERCAIGNE, J. WOLKE, I. NAERT and J. JANSEN, J. Biomed. Mater. Res. 41 (1998) 41.

    Article  CAS  Google Scholar 

  29. M. LI, N. MORRIS, S. MWENIFUMBO, M. KEIRSTEAD and W. SOBOYEJO, in Proceedings of SPIE 4830 (Osaka, 2002) 206.

  30. S. MWENIFUMBO, N. D. MORRIS, M. LI and W. O. SOBOYEJO, “Surface Engineering in Materials Science II” (San Diego, 2003) p. 141.

  31. A. M. P. TURNER, N. DOWELL, S. W. P. TURNER, L. KAM, M. ISAACSON, J. N. TURNER, H. G. CRAIGHEAD and W. SHAIN, J. Biomed. Mater. Res. 51 (2000) 430.

    Article  CAS  Google Scholar 

  32. N. D. SPENCER and M. TEXTOR, Surface Modification, Surface Analysis and Biomaterials. Materials in Medicine, edited by MD, Speidel and P Uggowitzer, (ETHZ, 1998).

  33. B. FENG, J. Y. CHEN, S. K. QI, L. HE, J. Z. ZHAO and X. D. ZHANG, J. Mater. Sci.: Mater. Med. 13 (2002) 457.

    Article  CAS  Google Scholar 

  34. B. FENG, J. WENG, B. C. YANG, J. Y. CHEN, J. Z. ZHAO, L. HE, S. K. QI and X. D. ZHANG Mater. Character., 49 (2003) 129.

    Article  CAS  Google Scholar 

  35. M. E. CHICUREL, C. S. CHEN and D. E. INGBER, Curr. Opin. Cell Biol. 10 (1998) 232.

    Article  CAS  Google Scholar 

  36. E. RUOSLAHTI and B. OBRINK, Exp. Cell Res. 227 (1996) 1.

    Article  CAS  Google Scholar 

  37. K. ANSELME, Biomater. 21 (2000) 667.

    Article  CAS  Google Scholar 

  38. P. CLARK, P. CONNOLLY, A. CURTIS, J. DOW and C. WILKINSON, Development 99 (1987) 439.

    CAS  Google Scholar 

  39. A. CURTIS and C. WILKINSON, Biomater. 18 (1997) 1573–1583.

    Article  CAS  Google Scholar 

  40. X. F. WALBOOMERS, W. MONAGHAN, A. CURTIS and J. A. JANSEN, J. Biomed. Mater. Res. 46 (1999) 212.

    Article  CAS  Google Scholar 

  41. P. CLARK, P. CONNOLLY, A. CURTIS, J. DOW and C. WILKINSON, Development 108 (1990) 635.

    CAS  Google Scholar 

  42. A. CURTIS and P. CLARK, CRC Rev. Biocomp. 5 (1990) 343.

    CAS  Google Scholar 

  43. J. MEYLE, K. GUTLIG and W. NISCH, J. Biomed. Mater. Res. 29 (1995) 81.

    Article  CAS  Google Scholar 

  44. A. GREEN, J. JANSEN, J. VAN DER WAERDEN and A. VON RECUM, J. Biomed. Mater. Res. 28 (1994) 647.

    Article  CAS  Google Scholar 

  45. H. G. CRAIGHEAD, S. W. TURNER, R. C. DAVIS, C. JAMES, A. M. PEREZ, P. M. ST. JOHN and M.S. ISAACSON. J. Biomed. Dev. 1(1) (1998) 49.

    CAS  Google Scholar 

  46. J. A. T. DOW, P. CLARK, P. CONNOLLY, A. S. G. CURTIS and C. D. W. WILKINSON, J. Cell. Sci. Suppl. 8 (1987) 55.

    CAS  Google Scholar 

  47. S. MWENIFUMBO, Cell/surface interactions and adhesion on biomedical and biomems surfaces: effects of laser micro-texturing and titanium coatings, A thesis of Princeton University (2004) p. 41.

  48. D. WANG, K. CHRISTENSEN, K. CHAWLA, G. XIAO, P. H. KREBSBACH and R. T. FRANCESCHI, J. Bone. Miner. Res. 14(6) (1999) 893.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. The Princeton Materials Institute and The Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, 08544, USA

    Steven Mwenifumbo, Jianbo Chen, Aboubaker Beye & Wolé Soboyejo

  2. Spectra-Physics, Inc., 1330 Terra Bella Ave., Mountain View, CA, 94043, USA

    Mingwei Li

  3. Department of Physics, Cheikh Anta Diop University, Dakar, Senegal

    Aboubaker Beye

Authors
  1. Steven Mwenifumbo
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  2. Mingwei Li
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  3. Jianbo Chen
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  4. Aboubaker Beye
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  5. Wolé Soboyejo
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Correspondence to Wolé Soboyejo.

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Mwenifumbo, S., Li, M., Chen, J. et al. Cell/surface interactions on laser micro-textured titanium-coated silicon surfaces. J Mater Sci: Mater Med 18, 9–23 (2007). https://doi.org/10.1007/s10856-006-0658-9

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  • DOI: https://doi.org/10.1007/s10856-006-0658-9

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