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The effect of hyaluronan combined with KI3 complex (Hyiodine wound dressing) on keratinocytes and immune cells

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Abstract

Hyiodine (high molecular weight hyaluronan combined with KI3 complex) is a new non-adhesive wound dressing which significantly improves the healing process. The aim of the study was to investigate the effects of Hyoidine on functional properties of isolated human keratinocytes and leukocytes, and on those of U937 and HL60 cell lines. While KI3 complex inhibited the viability and proliferation of the cells tested, Hyiodine did not have any significant effect. The expression of CD11b, CD62L and CD69 on PMNL, monocytes and lymphocytes, as well as the oxidative burst of blood neutrophils, were not changed. On the contrary, Hyiodine inhibited the PMA-activated oxidative burst and significantly increased the production of IL-6 and TNF-α by lymphocytes. It was concluded that hyaluronan content of Hyiodine reduces the toxic effect of KI3 complex on cells and speeds up the wound healing process by increasing the production of inflammatory cytokines.

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Abbreviations

CL:

chemiluminescence

HA:

hyaluronan

PMNL:

polymorphonuclear leukocytes

ROS:

reactive oxygen species

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Authors and Affiliations

  1. CPN spol. s.r.o., Dolni Dobrouc 401, 562 01, Dolni Dobrouc, Czech Republic

    Jana Frankova & Vladimir Velebny

  2. Institute of Biophysics AS CR, Kralovopolska 135, 612 65, Brno, Czech Republic

    Lukas Kubala, Milan Ciz & Antonin Lojek

Authors
  1. Jana Frankova
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  2. Lukas Kubala
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  3. Vladimir Velebny
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  4. Milan Ciz
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  5. Antonin Lojek
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Correspondence to Jana Frankova.

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Frankova, J., Kubala, L., Velebny, V. et al. The effect of hyaluronan combined with KI3 complex (Hyiodine wound dressing) on keratinocytes and immune cells. J Mater Sci: Mater Med 17, 891–898 (2006). https://doi.org/10.1007/s10856-006-0179-6

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  • DOI: https://doi.org/10.1007/s10856-006-0179-6

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