Abstract
Characterizations of hydroxyapatite (HA), biphasic calcium phosphate (BCP) and beta tricalcium phosphate (β-TCP) ceramic particles were carried out using X-ray diffusion (XRD), Scanning electron micrograph (SEM), Particle Sizer and Zeta potential analyzer. Competitive adsorption of bovine serum albumin (BSA) and lysozyme (LSZ) on the three calcium phosphates were investigated by polyacrylamide gel electrophoresis (PAGE) method. The results showed that HA, BCP and β-TCP ceramic particles with irregular shapes and similar size distributions all had negative surface net charges in pH7.4 phosphate buffered saline (PBS) solution and exhibited alike behaviors of BSA and LSZ adsorption. LSZ had higher affinity for calcium phosphate ceramics than BSA and its adsorption on them didn’t be almost influenced by the increasing of BSA concentration in the solution. Electrostatic interaction played an important role on the competitive adsorption of BSA and LSZ on the surface of calcium phosphate ceramic particles.
Similar content being viewed by others
References
H. YUAN, Z. YANG, J. D. DE BRUIJ, K. DE GROOT and X. ZHANG, Biomaterials 22 (2001) 2617
H. YUAN, Z. YANG, Y. LI, X. ZHANG, J. D. DE BRUIJN and K. DE GROOT, J. Mater. Sci. Mater. Med. 9 (1998) 723
X. ZHANG, H. YUAN and K. DE GROOT, In The 6th World Biomaterials Congress, Hawaii, May 2000 (USA: Society for Biomaterials, 2000)
H. YAMASAKI and H. SAKAI, Biomaterials 13 (1992) 308
H. YAMASAKI, Jpn. J. Oral Biol. 32 (1990) 190
H. YUAN, K. KURASHINA, J. D. DE BRUIJN, Y. LI, K. DE GROOT and X. ZHANG, Biomaterials 20 (1999) 1799
U. RIPAMONTI, Biomaterials 17 (1996) 31
Z. YANG, H. YUAN, W. TONG, P. ZOU, W. CHEN and X. ZHANG, Biomaterials 17 (1996) 2131
J. D. ANDRADE, In Surface and Interfacial Aspects of Biomedical Polymers (New York: Plenum Press, 1985) p. 1–80
W. NORDE, Cells Mat. 5 (1995) 97
D. A. PULEO and A. NANCI, Biomaterials 20 (1999) 2311
P. DUCHEYNE and Q. QIU, Biomaterials 20 (1999) 2287
A. EL-GHANNAM, P. DUCHEYNE and I. M. SHAPIRO, J. Orthop. Res. 17 (1999) 340
D. R. VILLARREAL, A. SOGAL and J. L. ONG, J. Oral Implantol. 24 (1998) 67
K. C. DEE, D. A. PULEO and R. BIZIOS, In An Introduction to Tissue-biomaterial Interactions (John Wiley & Sons, 2002), p. 37–52
C. HAYNES and W. NORDE, Colloid Surf. B 2 (1994) 517
T. BOIX, J. GOMEZ-MORALES, J. TORRENT-BURGUES, A. MONFORT, P. PULGDOMENECH and R. RODRIGUEZ-CLEMENTE, J. Inorg. Biochem. 99 (2005) 1043
K. KANDORI, K. MIYAGAWA and T. ISHIKAWA, J. Colloid Interf. Sci. 273 (2004) 406
A. KRAJEWSKI, A. PIANCASTELLI and R. MALAVOLTI, Biomaterials 19 (1998) 637
X. D. ZHU, H. S. FAN, C. Y. ZHAO, T. IKOMA, J. TANAKA, J. Y. CHEN and X. D. ZHANG, In Bioceramics 18, Kyoto, 2005 (Zurich-Uetikon: Trans Tech Publications Ltd, 2006), p. 73
G. YIN, Z. LIU, J. ZHAN, F. X. DING and N. J. YUAN, Chem. Eng. J. 87 (2002) 181
Q. L. LUO and J. D. ANDRADE, J. Colloid Interf. Sci. 200 (1998) 104
D. T. WASSELL and G. EMBERY, Biomaterials 17 (1996) 859
D. T. WASSELL, R. C. HALL and G. EMBERY, Biomaterials 16 (1995) 697
H. ZENG, K. K. CHITTUR and W. R. LACEFIELD, Biomaterials 20 (1999) 377
M. R. URIST, Science 150 (1965) 893
A. ROSENGREN, E. PAVLOVIC, S. OSCARSSON, A. KRAJEWSKI, A. RAVAGLIOLI and A. PIANCASTELLI, Biomaterials 23 (2002) 1237
E. C. I. VEERMAN, R. J. F. SUPPERS, C. KLEIN, K. DEGROOT and A. V. N. AMERONGEN, Biomaterials 8 (1987) 442
J. R. SHARPE, R. L. SAMMONS and P. M. MARQUIS, Biomaterials 18 (1997) 471
W. NORDE and C. E. GIACOMELLI, J. Biotechnol. 79 (2000) 259
J. BUIJS and V. V. HLADY, J. Colloid Interf. Sci. 190 (1997) 171
P. K. SMITH, R. I. KROHN, G. T. HERMANSON, A. K. MALLIA, F. H. GARTNER, M. D. PROVENZANO, E. K. FUJIMOTO, N. M. GOEKE, B. J. OLSON and D. C. KLENK, Anal. Biochem. 150 (1985) 76
U. K. LAEMMLI, Nature 227 (1970) 680
J. J. GRAY, Curr. Opinion Struct. Biol. 14 (2004) 110
Y. TIE, C. CALONDER and P. R. VAN TASSEL, J. Colloid Interf. Sci. 268 (2003) 1
K. KANDORI, T. SHIMIZU, A. YASUKAWA and T. ISHIKAWA, Colloid Surf. B 5 (1995) 81
Z. G. PENG, K. HIDAJAT and M. S. UDDIN, J. Colloid Interf. Sci. 271 (2004) 277
T. KAWASAKI, M. NIIKURA and Y. KOBAYASHI, J. Chromatogr. 515 (1990) 125
K. OHTA, H. MONMA and S. TAKAHASHI, J. Biomed. Mater. Res. 55 (2001) 409
M. VAN DER VEEN, W. NORDE, M. C. STUART, Colloids Surf. B Biointerfaces 35 (2004) 33
A. P. SERRO, M. BASTOS, J. C. PESSOA, B. SARAMAGO, J. Biomed. Mater. Res. Part A 70A (2004) 420
Z. G. PENG, K. HIDAJAT and M. S. UDDIN, Colloid Surf. B 33 (2004) 15
M. A. LOPES, F. J. MONTEIRO, J. D. SANTOS, A. P. SERRO and B. SARAMAGO, J. Biomed. Mater. Res. 45 (1999) 370
Acknowledgements
This work has been financially supported by National Basic Research Program of China (Contract No: G2005cb623901) and National Natural Science Foundation of China (Contract No: 50473051).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhu, X.D., Fan, H.S., Zhao, C.Y. et al. Competitive adsorption of bovine serum albumin and lysozyme on characterized calcium phosphates by polyacrylamide gel electrophoresis method. J Mater Sci: Mater Med 18, 2243–2249 (2007). https://doi.org/10.1007/s10856-006-0057-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10856-006-0057-2