Skip to main content
SpringerLink
Log in

Macroporous hydrogels based on 2-hydroxyethyl methacrylate

Part III Hydrogels as carriers for immobilization of proteins

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Four series of macroporous hydrogels based on crosslinked copolymers of 2-hydroxyethyl methacrylate (HEMA)—sodium methacrylate (MANa), copolymer HEMA—[2-(methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl), terpolymer HEMA—MANa—MOETACl and on a polyelectrolyte complex were used as carriers for immobilization of proteins, chicken egg white albumin and avidin. The adsorption capacity of the hydrogels for the two proteins, kinetics and pH dependence of albumin adsorption and desorption were studied. The morphology of the hydrogels with and without immobilized albumin was studied by low-vacuum scanning electron microscopy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. A. SURANI, Nature 414(2001) 122.

    Article  CAS  PubMed  Google Scholar 

  2. K. PRELLE, N. ZINK and E. WOLF, Anat. Histol. Embryol. 31 (2002) 169.

    Article  PubMed  Google Scholar 

  3. K. SMETANA JR., Biomaterials 14(1993) 1046.

    Article  CAS  PubMed  Google Scholar 

  4. K. SMETANA JR., J. VACÍ K, J. HAŠEK and M. ŠTOL, Biomimetics 1 (1992) 239.

    CAS  Google Scholar 

  5. J. LABSKÝ, B. DVOŘÁNKOVÁ, K. SMETANA JR., Z. HOLÍKOVÁ, L. BROŽ and H. J. GABIUS, Biomaterials 24(2003) 863.

    Article  PubMed  Google Scholar 

  6. K. SMETANA JR., J. VACÍK, D. SOUČKOVÁ, Z. KRČOVÁand J. ŠULC, J. Biomed. Mater. Res. 24 (1990) 463.

    Article  CAS  PubMed  Google Scholar 

  7. M. PŘÁDNÝ, P. LESNÝ, J. FIALA, J. VACÍK, M. ŠLOUF, J. MICHÁLEK and E. SYKOVÁ Collect. Czech. Chem. Commun. 68 (2003) 812.

    Article  Google Scholar 

  8. M. PŘÁDNÝ, P. LESNÝ, K. SMETANA JR., J. VACÍK, M. ŠLOUF, J. MICHÁLEK and E. SYKOVÁ J. Mater. Sci Mater. Med. in preparation.

  9. N. M. GREEN, Biochem. J. 94 (1965) 23.

    Google Scholar 

  10. M. M. BREDFORD, Anal. Biochem. 72 (1976) 248.

    CAS  PubMed  Google Scholar 

  11. J. H. HAN and C. H. LEO, Colloids Surf. 3 (1997) 131.

    Google Scholar 

  12. J. D. ANDRADE, in “Surface and Interfacial Aspects of Biomedical Polymers” (Plenum Press, New York and London, 1985) Vol. 2, p. 72.

    Google Scholar 

  13. R. C. BRUCH and H. B. WHITE, Biochemistry 21 (1982) 5334.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06, Prague 6, Czech Republic

    J. Micháalek, M. Přáadný, A. Artyukhov & M. šlouf

  2. D. I. Mendeleev University of Chemical Technology of Russia, Miusskaya Sq. 9, 125047, Moscow, Russia

    A. Artyukhov

  3. Center for Cell Therapy and Tissue Repair, Charles University, V Úvalu 84, Prague 6, Czech Republic

    J. Micháalek, M. Přáadný & K. Smetana Jr.

  4. Institute of Anatomy, 1st Faculty of Medicine, Charles University, 120 00, Prague 2, Czech Republic

    K. Smetana Jr.

Authors
  1. J. Micháalek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Přáadný
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Artyukhov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. šlouf
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. Smetana Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Micháalek.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Micháalek, J., Přáadný, M., Artyukhov, A. et al. Macroporous hydrogels based on 2-hydroxyethyl methacrylate. J Mater Sci: Mater Med 16, 783–786 (2005). https://doi.org/10.1007/s10856-005-2617-2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-005-2617-2

Keywords

Search

Navigation